Product introduction
This product offers a rapid and efficient method to isolate DNA from a wide range of samples, including:
- Tissues
- Cells
- Blood
- Saliva
- Swabs
- Blood Spots
- Semen
- And more!
The extracted DNA is ready for immediate use in downstream applications like:
- PCR (Polymerase Chain Reaction)
- qPCR (quantitative PCR)
- Southern Blotting
- Viral DNA detection
- Other DNA-based analyses
Specification
| Features | Specifications |
| Main Functions | Isolation of total DNA from tissue/blood /body fluid /swab /dry blood spots |
| Applications | PCR,qPCR, southern bolt and virus detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cells, blood, saliva, swabs, blood spots, semen, and other clinical samples |
| Sample amount | Solid tissue:1-10mg, Anticoagulant blood:200μ |
| Yield | 1 -15μg |
| Elution volume | ≥20μl |
| Time per run | 30 -60 minutes |
| Liquid carrying volume per column | 750μl |
| Binding yield of column | 100μg |
Principle
- Lysis and Digestion: Begin by lysing and digesting the sample using a specialized lysate and protease combination. This step facilitates the release of DNA into the lysate, preparing it for the purification process.
- Adsorption Column: Transfer the lysate mixture to an adsorption column. Here, the nucleic acid selectively adheres to the silica membrane, while proteins and other contaminants remain unadsorbed. This selective binding ensures the efficient removal of impurities.
- Filtration: Employ filtration to efficiently remove the unadsorbed proteins and other contaminants from the mixture. This step further enhances the purity of the nucleic acid sample.
- Thorough Washing: Perform a thorough washing step to eliminate any remaining proteins and impurities, ensuring the purity of the nucleic acid sample.
- Elution: Finally, elute the purified nucleic acid from the adsorption column using a low-salt buffer solution (10mm Tris, pH 9.0, 0.5mm EDTA). This gentle elution process preserves the integrity of the DNA, readying it for downstream applications.
Advantages
- Versatility: Suitable for a diverse range of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, and more. Whether you’re performing basic research or advanced molecular assays, our DNA meets your requirements.
- Efficiency: Accelerate your workflow with fast DNA extraction. Say goodbye to time-consuming processes like leukocyte separation, organic extraction, or ethanol precipitation. Our method streamlines the extraction process for swift results.
- Simplicity: Enjoy a straightforward extraction process. No complex steps or specialized equipment are required. Obtain all nucleic acids directly through simple digestion, saving you time and effort in the lab.
- Broad Applicability: Our DNA extraction method is versatile and adaptable. It can handle various sample types, including liquid samples, animal tissues, and cultured cells. Whether you’re working with blood, tissues, or cell cultures, our method delivers consistent results across different sample types.
Kit Contents
| Contents | VD3018 |
| Purification Times | 100 |
| 2ml Collection Tubes | 2×100 |
| Buffer ATL | 60 ml |
| Buffer AL | 60 m |
| Buffer GW1 | 44 m |
| Buffer GW2 | 50 m |
| Proteinase K | 60 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer AF | 15 m |
Storage and Stability
- Proteinase K Storage:
- Upon arrival, store at 2-8°C.
- Short-term storage (up to 12 weeks) at room temperature (15-25°C) is acceptable without affecting performance.
- Remaining Kit Components:
- Store at room temperature (15-25°C).
- Stable for at least 18 months under these conditions.
- Entire Kit Storage:
- Can be stored at 2-8°C.
- Ensure buffers are redissolved before use if stored at this temperature.
- Buffers should be at room temperature when used.
Experiment Data
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