Introduction
- Guanidine hydrochloride is a crucial component in nucleic acid extraction, selected after stringent screening and quality control.
- It acts as a powerful denaturant and ionizing agent, enhancing the solubility of hydrophobic molecules and denaturing proteins.
- Guanidine hydrochloride is effective in denaturing and inactivating various enzymes and proteins.
- In a 6 M guanidine hydrochloride solution, the “secondary structure” of proteins, maintained by intermolecular non-covalent interactions, is disrupted, causing changes in the tertiary structure.
- It is commonly used in nucleic acid extraction as a cleaning solution (> 3M), binding solution (4-6M) for protein removal, and lysate for genomic DNA and total RNA extraction.
- Unlike guanidine isothiocyanate, guanidine hydrochloride exhibits low absorbance in A260, A280, and A230, resulting in an ideal ratio of nucleic acid product A260/A230.
Specifications
| Features | Specification | |
| Basic content | Recommended application | Plasmid extraction, lysis Solution, or cleaning solution |
| CAS Numbers | 50-01-1 | |
| Formula | CH5N3.HCL | |
| Molecular Weight | 95.53 | |
| Content | 99.5% | |
| Level | Molecular Biology | |
| Appearance | White crystal will harden for a long time | |
| Transportation conditions | Room temperature | |
| Preservation conditions | Room temperature, dry, dark | |
| Impurity parameters | Moisture | ≤0.3% |
| Ash | ≤0.02% | |
| Water insoluble matter | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1% | |
| Ammonium cyanuric chloride | ≤0.01% | |
| Iron | ≤0.1PPM | |
| UV absorption value | Absorbance value @ 230 (6M) | ≤0.4 |
| Absorbance value @ 260 (6M) | ≤0.04 | |
| Absorbance value @ 280 (6M) | ≤0.01 | |
| Absorbance value @ 320 (6M) | ≤0.01 | |
| Nucleic acid extraction related | Plasmid extraction test | Adopt |
| Blood DNA extraction test | Adopt | |
| DNase test (2M) | Not detected | |
| RNase test (2M) | Not detected | |
| Purity of saturated solution (8M) | The 8m mother liquor is heated and dissolved. After dissolution, the insoluble matter is filtered with 3 layers of rapid filter paper. | |
| PH value (6M) | 5.0-5.5 |
Ordering information
| CAT.No. | Product Name | Package |
| C11101 | Guanidine Hydrochloride (Molecular Biology) | 5KG |
| C11102 | 25KG (5KG/Bag, 5Bags/Bucket) |
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