Introduction
This product highlights a versatile DNA extraction product suitable for various sample types:
- Tissues
- Cells
- Blood (including whole blood, plasma, serum, buffy coat)
- Saliva
- Swabs
- Blood Spots
- Semen
- Other clinical samples
Key Features:
- Rapid Extraction: Streamlined protocol ensures quick DNA isolation with minimal hands-on time.
- High-Quality DNA: Purifies total DNA (genomic, viral, mitochondrial) for reliable results in downstream applications like:
- PCR (Polymerase Chain Reaction)
- Quantitative PCR (qPCR)
- Southern Blotting
- Viral DNA detection
- Other DNA-based analyses
Specifications
| Features | Specifications |
| Main Functions | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| Applications | PCR, qPCR, southern bolt and virus detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cells, blood, saliva, swabs, blood spots, semen, and other clinical samples |
| Sample amount | Solid tissue: 1-10mg, Anticoagulant blood: 200µl |
| Yield | 1 – 15µg |
| Elution volume | <span “=””>≥20μl |
| Time per run | 30 – 60 minutes |
| Liquid carrying volume per column | 750µl |
| Binding yield of column | 100µg |
Principle
- This product is based on silica Column purification.
- The sample is lysed and digested with lysate and protease, and DNA is released into the lysate.
- Transfer to an adsorption column.
- Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration.
- After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Advantages
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
- Fast – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability – It can handle various liquid samples, animal tissues, and cultured cells
Kit Contents
| Contents | IVD3018 |
| Purification Times | 100 |
| HiPure DNA Mini Columns I | 100 |
| 2ml Collection Tubes | 2 x 100 |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer GW1 | 44 ml |
| Buffer GW2 | 50 ml |
| Proteinase K | 60 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer AE | 15 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case, buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
