This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from FFPE using high bind beads |
| Applications | RT-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Large quantities of solids |
| Sample amount | Appropriate |
| Elution volume | ≥50μl |
| Time per run | 30 – 120 minutes |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
- High yield – most optimal process, recovery up to 90%
- Economy – less than 50% of the price of Qiagen and other imported products
- Various operations – manual single tube/high-throughput or workstation
- High purity – OD 260/280 : 1.7-1.9, OD 260/230 :1.5-2.0
Kit Contents
| Contents | D632301B | D632302B |
| Purification Times | 48 Preps | 96 Preps |
| MagBind Particles | 1.1 ml | 2 x 1.1 ml |
| RNase A | 10 mg | 20 mg |
| Proteinase K | 24 mg | 48 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml |
| Buffer DPS | 60 ml | 100 ml |
| Buffer ATL | 15 ml | 30 ml |
| Buffer AL | 15 ml | 30 ml |
| Buffer BD* | 6 ml | 15 ml |
| Buffer BXW1* | 13 ml | 44 ml |
| Elution Buffer | 15 ml | 30 ml |
Storage and Stability
RNase A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Reviews
There are no reviews yet.