Product Overview
- Offers fast and easy purification methods for total DNA.
- Ensures reliable PCR and Southern Blotting results.
- Suitable for purification of various types of DNA: genomic, viral, mitochondrial.
- Can purify DNA from tissue and cultured cells.
Specifications
| Features | Specifications |
| Main Functions | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | Midi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples |
| Sample amount | 0.2-2 ml |
| Elution volume | ≥300μl |
| Time per run | ≤80 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 1mg |
Principles
- Utilizes silica column purification method.
- The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Advantages
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
- Fast – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability – handle a variety of liquid samples
Kit Contents
| Contents | D311302 | D311303 |
| Purification Times | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15ml Collection Tubes | 40 | 200 |
| Buffer ATL | 50 ml | 250 ml |
| Buffer AL | 50 ml | 250 ml |
| Buffer GW1* | 22 ml | 110 ml |
| Buffer GW2* | 12 ml | 50 ml |
| RNase A | 20 mg | 90 mg |
| Proteinase K | 100 mg | 440 mg |
| Protease Dissolve Buffer | 10 ml | 30 ml |
| Buffer AE | 20 ml | 120 ml |
Storage and Stability
- Proteinase K and RNase A should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect their performance.
- The remaining kit components can be stored at room temperature (15-25°C) and remain stable for at least 18 months under these conditions.
Experiment Data
Purchase Guide
| Name | CAT NO | Sample amount | Leukocyte protocol* | Colum type | Elutio volume | Average yield | Time per run |
| HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure Tissue&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 well plate | 3-8μg/200μl |
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