Product Overview
- Offers fast and easy methods for purification of total DNA.
- Ensures reliable PCR and Southern blotting.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Suitable for purification from tissue and cultured cells.
Specifications
| Features | Specifications |
| Main Functions | Isolation of total DNA from <25 mg tissue, culture cells, FTA card |
| Applications | PCR, Southern Bolt virus detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissue or cultured cells |
| Sample amount | Animal tissue : <25mg, Cultured cells : <5 x 106 |
| Elution volume | ≥30μl |
| Time per run | 30 – 60 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Principle
- Utilizes silica column purification method.
- The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Advantages
- Good repeatability – suitable for extracting high-yield DNA from different types of tissue samples
- High purity – can be used for downstream applications such as multiplex and quantitative PCR
- Fas t- simplified process, extracting several samples in 20 minutes (after digestion)
- Safety – no phenol or chloroform extraction, no alcohol precipitation
Kit Contents
| Contents | D312102 | D312103 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer ATL | 15 ml | 65 ml |
| Buffer DL | 15 ml | 65 ml |
| Buffer GW1 | 22 ml | 110 ml |
| Buffer GW2 | 12 ml | 50 ml |
| RNase A | 10 mg | 50 mg |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer AE | 15 ml | 60 ml |
| HiPure gDNA Mini Columns | 50 | 2 x 125 |
| 2 ml Collection Tubes | 100 | 5 x 100 |
Storage and Stability
- RNase A and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 24 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
- Alternatively, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
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