HiPure Stool DNA Kit

Stool Sample in Molecular Biology Research:

1. Utility and Applications:
   • Stool samples are increasingly utilized in animal molecular genetics, population ecology, behavioral ecology, and intestinal disease diagnosis.
   • They contain gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
2. Challenges:
   • Low content of exfoliated cells in the digestive tract and degradation of genetic material pose primary challenges.
   • Presence of inhibitors such as polysaccharides, bile acids, bile salts, bile pigments, digestive juices, and mucus can affect PCR-based molecular analysis.

Importance of DNA Extraction:

            Critical Role:

• • Selecting appropriate extraction methods is crucial for obtaining high-quality DNA and ensuring successful downstream detection of stool DNA.

HiPure Stool DNA Kit:

1. Solution Provided:
   • The HiPure Stool DNA Kit from Magen Company offers a promising solution.
   • Features good universality, high cost-effectiveness, high yield, and purification.
2. Unique Features:
   • Utilizes a unique solution system and inhibitory factor adsorbent to efficiently remove various impurities in stool samples.
   • The purified DNA can be directly used for PCR, quantitative PCR, and other applications.
3. Efficiency and Ease of Use:
   • Enables rapid and reliable isolation of high-quality genomic DNA from various stool samples.
   • Capable of processing up to 100 mg of stool samples within 60 minutes.
   • Combines reversible nucleic acid binding properties with spin column technology, eliminating PCR inhibiting compounds.
   • Suitable for PCR, restriction digestion, and next-generation sequencing applications.
4. Sustainability and Scalability:
   • Eliminates the need for organic extractions, reducing plastic waste and hands-on time.
   • Allows multiple samples to be processed in parallel, enhancing efficiency.

Details

Specifications

Principle

Stool DNA Extraction Process:

1. Homogenization and Lysis:
   • Stool sample is homogenized and treated in a specially formulated buffer containing detergent.
   • This step effectively lyses bacteria, yeast, and fungal samples present in the stool.
2. Contaminant Removal:
   • Our proprietary Absorber Solution is used to remove humic acid, proteins, polysaccharides, and other contaminants from the lysed sample.
3. Binding and Washing:
   • Adjusted binding conditions facilitate the attachment of DNA to a DNA Mini Column.
   • Two rapid wash steps are performed to remove trace contaminants, ensuring purity of the DNA.
4. Elution of Purified DNA:
   • Pure DNA is eluted from the column in a low ionic strength buffer.
   • The resulting purified DNA is ready for direct use in downstream applications without the need for further purification.

Advantages

• High purity – unique adsorbent can completely remove inhibitory factors
• High concentration – maximum extraction of total DNA from stool samples
• High recovery – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time

Kit Contents

Storage and Stability

Storage Instructions:

1. Proteinase K and Buffer PCI:
   • Upon arrival, store at 2-8°C.
   • Short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance.
2. Remaining Kit Components:
   • Store at room temperature (15-25°C).
   • Stable for at least 18 months under these conditions.
3. Entire Kit:
   • Can be stored at 2–8°C.
   • Buffers should be redissolved before use if stored at this temperature.
   • Ensure all buffers are at room temperature when used.