This product provides fast and easy methods for the purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from bacterial culture |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Gram negative or positive bacteria |
| Sample amount | Bacterial culture: 0.5-1.5ml |
| Elution volume | ≥60μl |
| Time per run | ≤60 minutes |
Principle
- This product utilizes high-binding magnetic particles for purification.
- Samples are lysed and digested using a lysate and Lysozyme.
- DNA is released into the lysate during this process.
- Magnetic particles and binding solution are added, allowing DNA to be adsorbed onto the surface of the particles while impurities such as proteins are not adsorbed.
- The adsorbed particles undergo washing with a washing solution to remove proteins and impurities.
- Ethanol is used for further washing to remove salts.
- Finally, DNA is eluted using Elution Buffer.
Advantages
- Fast: Multiple samples can be extracted within 60 minutes (after digestion), making the process time-efficient.
- High purity: Unique adsorbents ensure the complete removal of inhibitory factors, resulting in high-purity DNA extraction.
- High recovery: DNA can be recovered at the level of picograms (PG), indicating a high level of efficiency in the extraction process.
Kit Contents
| Contents | D513101 | D513102 | D513103 |
| Purification Times | 48 Preps | 96 Preps | 480 Preps |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml | 30 ml |
| Buffer SDS | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Buffer GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Storage and Stability
- MagPure Particles, Lysozyme, and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance.
- The remaining kit components can be stored dry at room temperature (15-25°C) and remain stable for at least 18 months under these conditions.
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