Introduction
The Kit integrates phenol/guanidine-based lysis and silica-membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate the lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg of animal, plant, and fungal samples, bacteria, cells, and other samples.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation of total RNA (including miRNA) from 100mg lipid tissue, tissue, cell, plant, and body fluids using columns and MagZol reagent |
| Applications | RT-PCR, qRT-PCR, Northern hybridization, second-generation sequencing |
| Purification method | Mini spin column |
| Purification technology | Silica technology, acid phenol/guanidine extraction technology (MagZol pretreatment technology) |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissue, muscle fiber-containing tissue, adipose tissue, cultured cells, lymphocytes, simple plants, and other biological samples |
| Sample amount | Animal tissue sample: 1-60mg (spleen ≤ 10mg) Cultured cells: 5 x 106 Plant leaves: 50-100mg |
| Yield | 2-200μg |
| Elution volume | ≥50μl |
| Time per run | ≤30 minutes（1-24 samples） |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Principle
Tissue samples (10-100mg) are homogenized in MagZol Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.
Advantages
- Efficient DNA removal – One-step RNA extraction can effectively remove genomic DNA
- High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Fast – several samples can be extracted in 30 minutes
- High applicability – samples including animals, plants, bacteria, cells, etc.
- High output – enables to processing of large samples and the yield can be up to 200μg
Kit Contents
| Contents | R413002 | D413003 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| MagZol Reagent | 60 ml | 270 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
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