Introduction
This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from FFPE tissue |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Products | RNA, miRNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | FFPE slice, FFPE embedded tissue |
| Sample amount | ≤6 slices |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
- Fast – several samples can be extracted in 120 minutes by column method
- Safe – no phenol chloroform extraction required
Kit Contents
| Contents | IVD3022 |
| Purification Times | 200 Preps |
| MagBind Particles | 4.5 ml |
| Proteinase K | 100 mg |
| Protease Dissolve Buffer | 6 ml |
| DNase I | 4 x 600 µl |
| DNase Buffer | 30 ml |
| Buffer DPS | 200 ml |
| Buffer FRL | 40 ml |
| Buffer AL | 40 ml |
| Buffer MW1* | 110 ml |
| Buffer MW2* | 2 x 50 ml |
| Nuclease Free Water | 30 ml |
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K and MagBind Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
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