HiPure Liquid RNA (miRNA) Kit

$124.00$547.00

Shipping USD 45 - Free over USD 300

Isolation total RNA from 0.25ml body fluids using column and MagZol LS reagent

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica-membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation of total RNA from 0.25ml body fluids using column and MagZol LS reagent
ApplicationsqPCR / RT-PCR, liquid or solid-phase chip analysis, hybridization, and SNP detection
Purification methodMini spin column
Purification technologySilica technology, acid phenol / guanidine extraction technology (MagZol pretreatment technology)
Process methodManual (centrifugation or vacuum)
Sample typeBlood, serum, plasma
Sample amount250μl
Elution volume20μl
Liquid carrying volume per column800µl
Binding yield of column100μg

Principle

0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100 µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.

Advantages

  • High quality – high purity total RNA can be directly used in various sensitive downstream applications
  • Fast – isolation of several samples can be completed in 40 minutes by using the column purification method
  • Safety – no phenol-chloroform extraction is required
  • Sensitive – direct lysis of blood, plasma, and other samples without separation of leukocytes

Kit Contents

ContentsR416302R416303
Purification Times50 Preps250 Preps
HiPure RNA Mini Columns50250
2ml Collection Tubes502 x 125
MagZol LS Reagent60 ml270 ml
Buffer RWC20 ml60 ml
Buffer RW2*20 ml2 x 50 ml
RNase Free Water10 ml30 ml

Storage and Stability

MagZol LS Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Additional information

Weight0.7 kg
size

HiPure Liquid RNA (miRNA) Kit 50 preps, HiPure Liquid RNA (miRNA) Kit 250 preps

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