This product supplies a simple and rapid extraction of total RNA from Blood, buffy coat, bone marrow, cell suspension, and other body fluids. The kit is based on superparamagnetic particle purification technology, with no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing, and so on.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from 300μl whole blood, bone marrow, buffy coat |
| Applications | RT-PCR, cDNA synthesis, second-generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Whole blood, buffy coat, bone marrow, cultured cells, lymphocytes, body fluid |
| Sample amount | ≤5×106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow |
| Yield | 1-30μg |
Principle
The Kit can be used for both manual extraction processes and automatic nucleic acid extraction machines. This Kit is suitable for extracting RNA from ≤5×106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow. This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. RNA/DNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally, RNA was eluted by Elution Buffer.
Advantages
- High purity – OD 260 / 280:1.9-2.0, OD 260 / 230:1.5-2.0
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – extraction without manual participation, saving time and effort
- High yield – nucleic acid recovery up to 95%
- General – samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.
Kit Contents
| Contents | R661101 | R661102 | R661103 |
| Purification Times | 48 Preps | 96 Preps | 480 preps |
| MagPure RNA Particles | 1.7 ml | 3.5 ml | 18 ml |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 1.8 ml | 5 ml | 15 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 µl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Buffer MLB | 40 ml | 80 ml | 350 ml |
| Buffer MCB* | 15 ml | 30 ml | 150 ml |
| Buffer MW1* | 22 ml | 44 ml | 220 ml |
| Buffer MW2* | 20 ml | 50 ml | 2 x 100 ml |
| RNase Free Water | 10 ml | 15 ml | 120 ml |
Storage and Stability
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 week, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
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