MagPure Particles N

$599.00$15,999.00

Shipping USD 45 - Free over USD 300

Magnetic beads for viral DNA/RNA extraction, plasmid, DNA/RNA Clean Up, 0.2~2um silica beads

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish-brown ferric oxide as the magnetic material. The surface of the bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads are commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and their principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast-speed silica magnetic beads. The core is ferric oxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.

Details

Specifications

FeaturesSpecifications
Concentration70 mg/ml
AppearanceSuspension of yellowish-brown particles
Surface functional groupSi-OH, Silanol
DispersibilityPolydisperse Amorphous
Particle size0.2-2 μm
Preservation conditionsRoom Temperature, valid for up to 2 years.

It is recommended to store at 2-8°C to prevent microbial growth.

Magnetic response speed~60 seconds
Settling velocity>10 minutes
High salt-mediated binding>2M guanidine isothiocyanate, DNA recovery up to 80%
Alcohol mediated binding2M guanidine hydrochloride/isopropanol (30%), and the recovery of DNA / RNA was as high as 85%
PEG8000 mediated bindingThe recovery of DNA/RNA was up to 85%
DNase/RNaseNot detected
DNA residue<1 ppm
Recommended applicationPlasmid extraction, gel DNA recovery, viral nucleic acid isolation

Principle

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol-mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with a digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles, and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form the DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, to achieve the purpose of purification.

Ordering information

CAT.No.Product NamePackage
C14110MagPure Particles N100 ml
C14111MagPure Particles N400 ml
C14112MagPure Particles N3 x 400 ml
C14113MagPure Particles N10 x 400 ml

Purchase Guide

FeaturesMagPure ParticlesMagPure Particles NMagPure Particles GMagPure Particles FMagBind Particles
Cat.No.C1410C1411C1412C1414C1413
Concentration100mg/ml70mg/ml40mg/ml50mg/ml10mg/ml
FormAmorphous and PorousAmorphous and PorousPorousAmorphousNonporous
Surface functionSi-OH, Silica BeadsSi-OH, Silica BeadsSi-OH, Silica BeadsSi-OH, Silica BeadsCOOH, Carboxyl Beads
DispersionPolydispersePolydisperseMonodisperseMonodisperseMonodisperse
Particle Size1.5-5μm0.2-2μm1-1.5μm0.2-1.5μm0.8-1μm
ColorBlackYellowish BrownDark BrownDark BrownYellowish Brown
Magnetic response15-30s~60s~30s20s120s
Settling Time (1ml)>5min>10min>3min>3min>2h
Usage (0.2ml Sample)20μl20μl20-30μl20-30μl20-30μl
DNA Recover Rate (only 4M GITC)>80%>80%>80%>80%0
DNA Recover Rate (10% PEG8000/NaCl)>85%>85%>85%>85%>90%
Recommended Use
  • gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.
  • Viral DNA/RNA Isolation
  • Agarose Gel DNA Purification
  • DNA/RNA Isolation from low nucleic acid content samples
  • Plasmid Isolation
  • DNA/RNA Clean Up
  • Circulating DNA Isolation
  • Viral Nucleic acid Isolation
  • gDNA Isolation
  • FFPE DNA/RNA Isolation
  • Plasmid extraction
  • gel DNA recovery
  • genomic DNA/RNA extraction
  • viral nucleic acid extraction
  • Circulating DNA extraction
  • DNA/RNA Clean Up and concentration
  • DNA/RNA Isolation from low nucleic acid content samples
  • Research immunoassays
  • The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.

Additional information

Weight0.75 kg
size

MagPure Particles N 100 ml, MagPure Particles N 400 ml, MagPure Particles N 3 x 400 ml, MagPure Particles N 10 x 400 ml

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