Introduction
The product is the core raw material for nucleic acid extraction after strict screening and quality control. Guanidine hydrochloride is a powerful denaturantor ionizing agent, which can promote the solubility of hydrophobic molecules and denature proteins. It is also an effective denaturant and inactivator of avariety of enzymes and proteins. In 6 M guanidine hydrochloride solution, the ordered “secondary structure” (maintained by intermolecular non covalent interactions, such as hydrogen bonds and hydrophobic bonds) of almost all proteins is destroyed and the peptide chain is stretched. The tertiary structure has changed. In the process of nucleic acid extraction, guanidine hydrochloride is often used as cleaning solution (> 3M), binding solution (4-6M) for protein removal, and lysate for genomic DNA and total RNA extraction. Different from guanidine isothiocyanate, guanidine hydrochloride has low absorbance in A260, A280 and A230, and the ratio of nucleic acid product A260 /230 is more ideal.
Details
Specifications
| Features | Specification | |
| Basic content | Recommended application | Plasmid extraction, lysis Solution or cleaning solution |
| CAS Numbers | 50-01-1 | |
| Formula | CH5N3.HCL | |
| Molecular Weight | 95.53 | |
| Content | 99.5% | |
| Level | Molecular Biology | |
| Appearance | White crystal, will harden for a long time | |
| Transportation conditions | Room temperature | |
| Preservation conditions | Room temperature, dry, dark | |
| Impurity parameters | Moisture | ≤0.3% |
| Ash | ≤0.02% | |
| Water insoluble matter | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1% | |
| Ammonium cyanuric chloride | ≤0.01% | |
| Iron | ≤0.1PPM | |
| UV absorption value | Absorbance value @ 230 (6M) | ≤0.4 |
| Absorbance value @ 260 (6M) | ≤0.04 | |
| Absorbance value @ 280 (6M) | ≤0.01 | |
| Absorbance value @ 320 (6M) | ≤0.01 | |
| Nucleic acid extraction related | Plasmid extraction test | Adopt |
| Blood DNA extraction test | Adopt | |
| DNase test (2M) | Not detected | |
| RNase test (2M) | Not detected | |
| Purity of saturated solution (8M) | The 8m mother liquor is heated and dissolved. After dissolution, the insoluble matter is filtered with 3 layers of rapid filter paper. | |
| PH value (6M) | 5.0-5.5 |
Ordering information
| CAT.No. | Product Name | Package |
| C11101 | Guanidine Hydrochloride (Molecular Biology) | 5KG |
| C11102 | 25KG (5KG/Bag, 5Bags/Bucket) |
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