Introduction
The product is the core raw material for nucleic acid extraction after strict screening and quality control. Guanidine thiocyanate is commonly used as lysate denaturant, and agarose gel sol in the process of nucleic acid extraction. Large 2m guanidine isothiocyanate can mediate the adsorption of isodna on glass fiber filter membranes or silica magnetic beads. Different from guanidine hydrochloride, guanidine isothiocyanate has a high absorbance value in A230, 0.5% (w / V) guanidine isothiocyanate (~4mm), and its A260 / A230 is less than 0.5. For the nucleic acid obtained by using this lysate, its A260 / 230 will be poor in low-concentration nucleic acid samples, but it will not affect PCR, RT-PCR, and second-generation sequencing.
Details
Specifications
| Features | Specification | |
| Basic content | Recommended application | Agarose Gel DNA Purification, DNA/RNA Isolation (Lysis Buffer) |
| CAS Numbers | 593-84-0 | |
| Formula | CH5N3•HSCN | |
| Molecular Weight | 118.6 | |
| Content | 99.5% | |
| Level | Molecular Biology | |
| Appearance | White powder to crystal | |
| Transportation conditions | Room temperature, dry | |
| Preservation conditions | Avoid light | |
| Impurity parameters | Moisture | ≤0.3% |
| Ash | ≤0.02% | |
| Water insoluble matter | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1% | |
| Ammonium cyanuric chloride | ≤0.01% | |
| Iron | ≤0.1PPM | |
| UV absorption value | Absorbance value @ 230 (6M) | ≤20 |
| Absorbance value @ 260 (6M) | ≤25 | |
| Absorbance value @ 280 (6M) | ≤0.3 | |
| Absorbance value @ 320 (6M) | ≤0.01 | |
| Nucleic acid extraction related | Gel DNA Purification | Adopt |
| RNA Isolation | Adopt | |
| DNase test (1M) | Not detected | |
| RNase test (1M) | Not detected | |
| Purity of saturated solution (6M) | Endothermic during dissolution, no precipitation at room temperature after heating and dissolution, clear and colorless (no filtration) | |
| PH value (1M) | 5.0-7.0 |
Ordering information
| CAT.No. | Product Name | Package |
| C11103 | Guanidine Thiocyanate (Molecular Biology) | 5KG |
| C11104 | 25KG (5KG/Bag, 5Bags/Bucket) |
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