Introduction
- Product Quality: Subject to stringent screening and quality control measures, the product serves as the fundamental material for nucleic acid extraction, ensuring reliability and efficiency.
- Triton X-100 Properties: Triton X-100 is a non-ionic surfactant featuring both hydrophilic and hydrophobic ends. Its stability in aqueous solutions, resistance to strong electrolytes, and ability to form soluble complexes with lipids, such as phospholipids, contribute to its versatile functionality.
- Membrane Interaction: The hydrophobic end of Triton X-100 interacts with the hydrophobic regions of membrane proteins, facilitating their dissolution in solution. This property aids in increasing cell membrane permeability and disrupting cell membranes, enhancing nucleic acid release.
- Lipid Dissolution: Triton X-100 can dissolve lipids, further augmenting cell membrane permeability and promoting cell membrane rupture.
- Synergy with SDS: When combined with SDS, Triton X-100 synergistically enhances cell lysis and disrupts the surface membrane structure of enveloped viruses. This synergy facilitates efficient nucleic acid release from cells.
- Antagonistic Effect: Triton X-100 counteracts the residual effects of SDS, inhibiting any adverse impact on subsequent processes. This antagonistic property ensures the smooth progression of nucleic acid extraction procedures.
Specifications
| Features | Specification | |
| Basic content | Recommended application | DNA / RNA extraction, viral nucleic acid extraction |
| CAS Numbers | 9002-93-1 | |
| Formula | C18H30O3 | |
| Level | Molecular Biology | |
| Appearance | White transparent or translucent liquid | |
| Transportation conditions | Room temperature | |
| Preservation conditions | Room temperature | |
| Quality guarantee period | 2 years | |
| Impurity parameters | Smell | Nothing |
| Acid value (mgKOH/g) | ≤1.0% | |
| Saponification value (mgKOH/g) | 45-60 | |
| Relative viscosity | 1.2-1.5 | |
| Moisture | ≤1.0% | |
| Iron | ≤5ppm | |
| Potassium | ≤0.05% | |
| Heavymetal ion | ≤5ppm | |
| UV absorption value | Absorbance value @ 230 (1%) | ≤30 |
| Absorbance value @ 260 (1%) | ≤15 | |
| Absorbance value @ 280 (1%) | ≤30 | |
| Absorbance value @ 320 (1%) | ≤0.1 | |
| Nucleic acid extraction related | DNA extraction test | Adopt |
| Virus total nucleic acid test | Adopt | |
| DNase test (5%) | Not detected | |
| RNase test (5%) | Not detected | |
| Impurity analysis (100%) | 60 degrees full solution |
Ordering information
| CAT.No. | Product Name | Package |
| C11209 | Triton X-100, for molecular biology | 4000 ml |
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