Product introduction：
- Support Material: Agarose serves as a common support material in gel electrophoresis.
- Purity Impact: The purity of agarose directly influences the resolution of DNA bands and the clarity of electrophoresis results.
- Preparation: Agarose, a high-purity form of agar, is typically prepared as a gel with concentrations ranging from 0.5% to 2%.
- Applications: Agarose gel electrophoresis is utilized for the separation and identification of nucleic acids, including DNA profiling and DNA restriction enzyme mapping.
- Visualization: Nucleic acid fluorescent dyes are added to the gel, and DNA bands are visualized using a gel imaging scanner post-electrophoresis.
Product contents：
| Components | DA003-02（100g） |
| Agarose | 100g |
Quality Control:
| Parameter | Specification |
|---|---|
| Gel Strength (1% gel) | > 1200g/cm² |
| Electroendosmosis (EEO) | < 0.15 |
| Sulfate | ≤ 0.15% |
| Gel Temperature (1.5% gel) | 35-37℃ |
| Melting Temperature (1.5% gel) | 87-89℃ |
| Moisture | ≤ 10% |
Absence of Nucleases.
Usage Instructions:
- Depending on the size of the target nucleic acid fragments and the type of electrophoresis buffer, determine the required agarose concentration:
| Agarose concentration | Ideal linear resolution range（bp） | |
| 1× TAE | 1× TBE | |
| 0.6% | 1200-15000 | 1200-12000 |
| 0.8% | 1000-10000 | 1000-12000 |
| 1.0% | 200-10000 | 100-10000 |
| 1.2% | 100-8000 | 100-5000 |
| 1.5% | 100-5000 | 50-3000 |
| 2.0% | 50-3000 | 50-3000 |
| 2.5% | 50-3000 | 50-2000 |
- Agarose Gel Preparation (Horizontal Gel Electrophoresis Example):
- Based on the amount of gel needed and the desired agarose concentration, measure an appropriate volume of electrophoresis buffer (TAE or TBE) and pour it into a triangular flask.
Note: The buffer used for gel preparation should be the same as the electrophoresis buffer.
- Accurately weigh the agarose and carefully add it to the triangular flask. Cover the flask opening with parchment paper and heat it in a microwave oven to dissolve the agarose. Heat the solution until it boils, then wearing heat-resistant gloves, gently swirl the flask. Repeat this process several times until the agarose is completely dissolved.
Note: Use multiple short boiling steps during the agarose dissolution to avoid overheating and boiling over. Ensure the agarose is fully dissolved to avoid blurry electrophoresis results.
- Add nucleic acid dye to the fully dissolved agarose solution.
- Pour the agarose solution into the gel mold, then insert the comb at the appropriate position. The gel thickness is usually between 3-5mm.
- Let the gel solidify at room temperature (approximately 30 minutes to 1 hour) and then place it in the electrophoresis apparatus for electrophoresis.
Precautions:
- Beware of boiling during the agarose dissolution process to prevent scalding.
- DA003-01 is a domestic proprietary brand, and DA003-02 is an imported reagent.
- Please wear gloves, especially when using fluorescent dyes with carcinogenic properties (such as ethidium bromide) for gel nucleic acid staining.
- If the prepared gel is not used immediately, store it submerged in electrophoresis buffer (TAE or TBE) to avoid gel drying out.
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