Migi (Dog) CDV/CPV/CAV Ab Rapid Quantitative Test Kit

Notice: You need an Immunofluorescence Analyzer to run the tests with this kit

Contact us for the equipment and test kits combo wholesale

SPECIFICATION

10 Test/Box

Cat.No.:MG-CA-R032

INTENDED USE

The CDV/CPV/CAV Ab test is a fluorescence immunoassay used along with an Immunofluorescence Analyzer for quantitative determination of the antibody concentration of panleukopenia virus, distemper virus, and adenovirus in serum or plasma specimens. The test may offer a reference of antibody titer levels after pets’ immune vaccine and determine whether the pets need to be immune again. Another use of the test is an aid in the diagnosis and monitoring of the infection of canine panleukopenia virus, canine distemper virus, and canine adenovirus.

For in vitro diagnostic use only. For professional use only

TEST PRINCIPLE

1. This test employs a quantitative double antigen sandwich fluorescence immunoassay technique.
2. The fluorescent signal intensity reflects the amount of CDV-Ab, CPV-Ab, CAV-Ab captured. The concentration is expressed as Tu/ml.

MATERIAL

STORAGE AND STABILITY

1. Store the test kit at 4～30℃ up to the expiration date.
2. The test should operate at 18 ~ 28℃ after the Test Device opening.
3. Once the pouch is opened, the test should be performed within 30 minutes.

SPECIMEN COLLECTION AND PREPARATION

The test can be performed with serum or plasma.

1. Separate serum or plasma (EDTA anticoagulant is recommended) from blood within 3 hours after blood collection. If a specimen appears to be severely hemolyzed, another specimen should be obtained and tested.

2. Optimally, the test should be performed immediately after the specimen collection. If the test cannot be performed within 3 hours after blood collection, store the specimen at 2 ~ 8℃ for no longer than 72 hours. For long-term storage, specimens shall be kept below -20℃.

Bring all materials to room temperature before use. Frozen specimens must be completely thawed and mixed well before testing. Specimens should not be frozen and thawed repeatedly. Only clear, non-hemolyzed specimens can be used.

TEST PROCEDURE

1. Preparation:
   • Set a Test Device on a clean, level horizontal table.
   • Insert ID Chip into the meter, and click “Read ID chip.” Make sure the Test Device lot No. matches with ID Chip No.
2. Sample Preparation:
   • Pipette 20μl of prepared sample into CDV/CPV/CAV Ab Sample Buffer.
   • Gently mix well. Avoid vigorous agitation and foaming.
3. Run the Test:
   • Choose between Standard Test (a) or Quick Test (b).

(a) Standard Test: * Pipette 100 μl of mixed sample into the sample well (S) of the CPV-Ab Test Device. * Click “Standard Test,” insert the CPV-Ab Test Device into the meter holder. * Select sample type “Serum/Plasma.” The meter will start a countdown and read the result automatically. * Repeat for CDV-Ab and CAV-Ab Test Devices, clicking “Start Test” for each after inserting them into the holder.

(b) Quick Test: * Pipette 100 μl of mixed sample into the sample well (S) of the CPV-Ab Test Device. * Click “Quick Test,” to start the timer immediately. * Leave the CPV-Ab Test Device at room temperature (18℃-28℃) for 10 minutes. * Insert the CPV-Ab Test Device into the meter holder and click “Start Test.” Select sample type “Serum/Plasma.” * Repeat for CDV-Ab and CAV-Ab Test Devices, inserting them in turn and clicking “Start Test” for each.

4. Results:
   • Results are displayed on the main screen.
   • Print automatically or print by clicking “Print.”

QUALITY CONTROL

Each CDV/CPV/CAV Ab Rapid Quantitative Test contains an internal control for routine quality control requirements. This internal quality control is performed each time a patient sample is tested, If an invalid result from the internal control occurs, the meter will display an error message, indicating that another test should be conducted.

INTERPRETATION

Reference range of CDV/CPV/CAV Ab in canine serum or plasma.

1. Detection range:

① CPV-Ab: 4.5-640 Tu/ml

② CDV-Ab: 5.0-640 Tu/ml

③ CAV-Ab: 4.0-640 Tu/ml

2. Reference range:

Each laboratory should establish a reference range that is representative of the population to be evaluated

WARNINGS AND LIMITATIONS OF THE PROCEDURE

1. This kit is for in vitro diagnostic use only.
2. Inspect the packaging and labels before use. Do not use it if the pouch is broken, torn, or not well sealed, or if the vial looks damaged or leaked.
3. Do not use the test device beyond the expiration date.
4. One pipette tip should be used for one specimen only.
5. There is the possibility that factors such as technical or procedural errors, as well as additional substances in blood specimens that are not listed in the text, may interfere with the test and cause erroneous results.