Building a Bird Gender and DNA Test Mini Lab

Introduction

For bird breeders, especially large-scale breeding farms for parrots, pigeons, etc., the construction of a small molecular detection laboratory can quickly detect the sex of fledglings by detecting genes, and can also detect bird viruses during the breeding process and achieve early prevention.

How to Build a Mini Bird DNA Test Lab?

The construction of a small DNA detection laboratory does not require a large investment in laboratory construction, but a relatively clean space is sufficient. The instruments and equipment required for a small DNA detection laboratory are the most basic and necessary, and the quantity is not large.

Here, I have compiled a list of the equipment and reagents needed to build a small-scale avian DNA detection laboratory, along with reference prices.

Real-time PCR method to detect the sex identification protocol of parrots, pigeons, and jade birds – SanshiBio ( price for entire system only)
No.	Product name	Model	Price($)	Quantity	Remark
1	Real-time fluorescent quantitative PCR	X4	18286	1	4 channels 、96 wells
2	Rapid Genomic DNA Extraction Kit forAnimals (for PCR analysis)	200T	50	1
3	Gender detection reagent	100T	278	2
4	Mini centrifuge	MINI10K+C	214	1
5	Mini mixer	MIX-25P	214	1
6	Pipette	10ul	29	1
7	Pipette	100ul	29	1
8	Pipette	1000ul	29	1
9	Pipette tips	10ul	2	1
10	Pipette tips	100ul	2	1
11	Pipette tips	1000ul	3	1
12	0.2ml eight-row tube	0.2ml	34	1
13	2.0ml centrifuge tubes	500 pcs	6	1
14	Disposable latex gloves		9	1
15	Icebox	0.2ml*96	21	1
16	1.5ml/2.0ml EP tube rack		1	1
17	0.2 ml EP tube rack	96 wells	5	5
18	Nucleic acid contamination scavenger NC	500ml	86	1
19	Scissors		0		Prepare yourself
20	Tweezers		0		Prepare yourself
21	Desktop trash can		0		Prepare yourself
22	Disposable garbage bags		0		Prepare yourself
23	Disinfect alcohol		0		Prepare yourself
24	paper towel		0		Prepare yourself
25	Cryogenic freezer		0		Prepare yourself
Optional Instrument
1	Hot lid metal bath	DTC-100H	952
2	Hot lid metal bath	DTH-100H	640

As we can see from the list, the most expensive part is the “Real-time fluorescent quantitative PCR machine”. However, if you do not have enough budget and you do not want to test 1000 birds a day, you may choose a smaller qPCR machine that is much cheaper less than 10k USD.

What size of space is needed for laboratory construction?

For avian detection laboratories, a large area is not required for construction. Generally, a maximum of about 30 square meters is sufficient. If the sample size for detection is small, a smaller space can be used.

How many people are needed for avian gender detection?

At least one person is required for avian gender test experiments. Efficiency increases with two people; one can handle sample preparation while the other prepares for the detection process.

What level of cleanliness is required for laboratory construction?

qPCR bird gender and disease test experiments do not have very high cleanliness requirements. Daily cleanliness, minimal dust, and dry conditions are sufficient.

Can qPCR equipment from other brands be used with SanshiBio’s reagents?

Yes, other brands of detection equipment can be used as long as they are operated according to the requirements of the detection reagents.

How long does it take to conduct one avian gender detection?

In one avian gender detection, the processing time for one set of samples (96T) is approximately 30 minutes, while qPCR detection from preparation to result output takes about 40-50 minutes.

How many avian gender tests can be done in one testing session?

A set of samples can accommodate up to 96 samples, with a general recommendation of 90 samples. Smaller channel devices can typically test around 48 samples.

What other experiments can be conducted in the laboratory besides avian gender detection?

Besides avian gender testing, a complete set of PCR detection equipment allows for various other test experiments using different reagents.

Does SanshiBio offer technical guidance?

Yes, the company provides comprehensive laboratory operation training. Additionally, they welcome on-site learning in China, which typically takes 2-3 days. Guidance is also available via direct video communication.

How To Use the Mini Bird DNA Test Lab?

Here is guidance on laboratory equipment and reagent operations, including instructional videos for reference and learning：

https://drive.google.com/drive/folders/14HkhVCirVzbfKqGlOopRt-jkOPLhvsum?usp=drive_link

Summary