Einführung
Das Produkt ist nach strenger Prüfung und Qualitätskontrolle der Hauptrohstoff für die Nukleinsäureextraktion. Guanidine thiocyanate is commonly used as lysate denaturant, and agarose gel sol in the process of nucleic acid extraction. Large 2m guanidine isothiocyanate can mediate the adsorption of isodna on glass fiber filter membranes or silica magnetic beads. Different from guanidine hydrochloride, guanidine isothiocyanate has a high absorbance value in A230, 0.5% (w / V) guanidine isothiocyanate (~4mm), and its A260 / A230 is less than 0.5. For the nucleic acid obtained by using this lysate, its A260 / 230 will be poor in low-concentration nucleic acid samples, but it will not affect PCR, RT-PCR, and second-generation sequencing.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikation | |
| Grundlegende Inhalte | Empfohlene Anwendung | Agarose Gel DNA-Reinigung, DNA/RNA Isolation (Lysepuffer) |
| CAS-Nummern | 593-84-0 | |
| Formel | CH5N3•HSCN | |
| Molekulargewicht | 118.6 | |
| Content | 99.5% | |
| Ebene | Molekularbiologie | |
| Aussehen | White powder to crystal | |
| Transportbedingungen | Zimmertemperatur, trocken | |
| Aufbewahrungsbedingungen | Avoid light | |
| Verunreinigungsparameter | Feuchtigkeit | ≤0.3% |
| Ash | ≤0.02% | |
| Water insoluble matter | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1% | |
| Ammonium cyanuric chloride | ≤0.01% | |
| Iron | ≤0.1PPM | |
| UV-Absorptionswert | Absorptionswert @ 230 (6M) | ≤20 |
| Absorptionswert @ 260 (6M) | ≤25 | |
| Absorptionswert @ 280 (6M) | ≤0.3 | |
| Absorptionswert @ 320 (6M) | ≤0,01 | |
| Im Zusammenhang mit der Extraktion von Nukleinsäuren | Gel DNA Purification | Adoptieren |
| RNA Isolation | Adoptieren | |
| DNase-Test (1M) | Nicht erkannt | |
| RNase-Test (1M) | Nicht erkannt | |
| Purity of saturated solution (6M) | Endothermic during dissolution, no precipitation at room temperature after heating and dissolution, clear and colorless (no filtration) | |
| PH Wert (1M) | 5.0-7.0 |
Bestellinformationen
| Katze nein. | Produktname | Paket |
| C11103 | Guanidine Thiocyanate (Molekularbiologie) | 5KG |
| C11104 | 25KG (5KG/Bag, 5Bags/Bucket) |
Bewertungen
Es gibt noch keine Bewertungen.