Einführung
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, usw. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, usw.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total RNA from bacteria culture |
| Anwendungen | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Bacteria culture |
| Probenmenge | Bacteria: <10^9 |
| Elutionsvolumen | ≥30μl |
| Zeit pro Lauf | ≤40 minutes |
| Flüssigkeitstransportvolumen pro Säule | 800µl |
| Bindungsausbeute der Säule | 100µg |
Prinzip
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as Guanidin hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Vorteile
- Schnell – Es können mehrere Proben entnommen werden 30 Protokoll
- Hohe Reinheit – the purified RNA can be directly used in various downstream applications
- High recovery – RNA can be recovered at the level of PG
- Good repeatability – silica gel column purification technology can obtain ideal results every time
- Breites Spektrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components – the kit contains carried lysozyme and glass beads
Inhalt des Kits
| Inhalt | R418102 | R418103 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| HiPure RNA Mini-Säulen | 50 | 250 |
| 2ml-Sammelröhrchen | 50 | 250 |
| Glass Beads (0.1-0.2mm) | 20 G | 90 G |
| Lysozyme | 90 mg | 400 mg |
| Protease-Auflösungspuffer | 5 ml | 15 ml |
| Buffer STE | 10 ml | 30 ml |
| MagZol Reagent | 60 ml | 270 ml |
| Puffer RW2* | 20 ml | 2 X 50 ml |
| RNase-freies Wasser | 10 ml | 30 ml |
Lagerung und Stabilität
MagZol Reagent and Lysozyme should be stored at 2-8°C upon arrival. Die restlichen Kit-Komponenten können trocken bei Raumtemperatur gelagert werden (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen. Das gesamte Kit kann bei 2–8 °C gelagert werden, In diesem Fall sollten die Puffer jedoch vor der Verwendung erneut aufgelöst werden. Stellen Sie sicher, dass alle Puffer bei der Verwendung Raumtemperatur haben.
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