The Kit provides fast purification of high-quality RNA from whole blood, Zellen, and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolierung der Gesamt-RNA aus 1–1,5 ml Vollblut |
| Anwendungen | qPCR / RT-PCR, liquid or solid-phase chip analysis, Hybridisierung, and SNP detection |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie, acid phenol/Guanidin extraction technology (MagZol pretreatment technology) |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Fresh or frozen blood, mammalian blood |
| Probenmenge | ≤1.5 ml whole blood |
| Ertrag | 2-100μg |
| Elutionsvolumen | ≥20μl |
| Flüssigkeitstransportvolumen pro Säule | 800µl |
| Bindungsausbeute der Säule | 100μg |
Prinzip
The Kit simplifies the isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells are collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.
Vorteile
- Gute Qualität – high purity total RNA can be directly used in various sensitive downstream applications
- Schnell – isolation of several samples can be completed in 20 minutes by using the column purification method
- Sensitive – RNA can be purified at the level of PG
Inhalt des Kits
| Inhalt | R416102 | R416103 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| HiPure DNA Mini-Säulen | 50 | 250 |
| HiPure RNA Mini Columns I | 50 | 250 |
| 2ml-Sammelröhrchen | 100 | 500 |
| 10 x Buffer RBC | 50 ml | 3 X 100 ml |
| RTL Lysis Buffer | 50 ml | 250 ml |
| Puffer RW1 | 50 ml | 250 ml |
| Puffer RW2* | 20 ml | 2 X 50 ml |
| RNase-freies Wasser | 10 ml | 30 ml |
Lagerung und Stabilität
HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen. Stellen Sie sicher, dass alle Puffer bei der Verwendung Raumtemperatur haben. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.
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