Einführung

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

Einzelheiten

Spezifikationen

Prinzip

Biological samples are first lysed and homogenized in a highly denaturing Guanidin isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.

Vorteile

• Gute Qualität – one step RNA-Extraktion reagent combined with silica gel column can obtain the highest concentration
• Schnell – Es können mehrere Proben entnommen werden 40 Protokoll
• High applicability – samples including animals, Pflanzen, Bakterien, Zellen, usw.
• High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
• Sicher – no phenol chloroform extraction and no use of Trizol reagent

Inhalt des Kits

Lagerung und Stabilität

Proteinase K should be stored at 2-8°C upon arrival. Jedoch, kurzfristige Lagerung (bis zu 12 Wochen) bei Raumtemperatur (15-25°C) hat keinen Einfluss auf ihre Leistung. Die restlichen Kit-Komponenten können bei Raumtemperatur gelagert werden (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen.