HiPure zirkulierendes DNA-Kit

Einführung

• Target: Short DNA fragments (<1000bp) in the blood (tumor markers, fetal DNA) or urine.
• Efficiency: Purifies circulating nucleic acids from human biofluids (Plasma, Serum, urine).
• Anwendungen: Extracted DNA is suitable for approved clinical diagnostic tests.

Vorteile:

• Optimized for short DNA analysis.
• Works with various human fluids.
• A larger format processes more samples (potentially higher DNA yield).
• Directly usable in clinical tests.

This Midi Kit empowers researchers and clinical labs for applications in oncology, NIPT, and liquid biopsies.

Spezifikationen

Prinzip

This product offers a streamlined method for isolating DNA using silica column technology:

1. Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
2. Binding to Silica: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.
3. Waschen: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.
4. Elution: A low-salt buffer is used to elute (freigeben) the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.

Key Advantages:

• Efficient Purification: Silica column technology offers a fast and reliable process for isolating DNA from complex biological samples.
• High-Purity DNA: The selective binding properties of the silica membrane ensure the purification of high-quality DNA, suitable for various downstream applications (PCR, usw.).
• Vereinfachter Arbeitsablauf: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

Vorteile

• Hohe Ausbeute – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration – low elution volume, ensuring high nucleic acid concentration
• Hohe Reinheit – low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery – DNA can be recovered at the level of PG by silica gel column purification

Inhalt des Kits

Lagerung und Stabilität

• Proteinase K & RNA Carrier: Fridge (2-8°C) for best results.
• Other Kit Components: Zimmertemperatur (15-25°C) is good.
• Storing Cold? Warm buffers up before use.

Experimentdaten