Einführung
- Target: Short DNA fragments (<1000bp) in the blood (tumor markers, fetal DNA) or urine.
- Efficiency: Purifies circulating nucleic acids from human biofluids (Plasma, Serum, urine).
- Anwendungen: Extracted DNA is suitable for approved clinical diagnostic tests.
Vorteile:
- Optimized for short DNA analysis.
- Works with various human fluids.
- A larger format processes more samples (potentially higher DNA yield).
- Directly usable in clinical tests.
This Midi Kit empowers researchers and clinical labs for applications in oncology, NIPT, and liquid biopsies.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation circulating DNA from 1-5ml plasma, Serum, and body fluids using vacuum protocol |
| Anwendungen | qPCR, liquid or solid chip analysis, hybridization SNP detection, usw. |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (vacuum) |
| Beispielstyp | Serum, Plasma, and other cell-free fluid samples |
| Probenmenge | 1-5ml |
| Elutionsvolumen | ≥50μl |
| Zeit pro Lauf | ≤60 minutes |
| Flüssigkeitstransportvolumen pro Säule | 4ml |
| Bindungsausbeute der Säule | 1mg |
Prinzip
This product offers a streamlined method for isolating DNA using silica column technology:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
- Binding to Silica: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.
- Waschen: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.
- Elution: A low-salt buffer is used to elute (freigeben) the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.
Key Advantages:
- Efficient Purification: Silica column technology offers a fast and reliable process for isolating DNA from complex biological samples.
- High-Purity DNA: The selective binding properties of the silica membrane ensure the purification of high-quality DNA, suitable for various downstream applications (PCR, usw.).
- Vereinfachter Arbeitsablauf: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Vorteile
- Hohe Ausbeute – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
- High concentration – low elution volume, ensuring high nucleic acid concentration
- Hohe Reinheit – low alcohol binding method, completely removing inhibitor and protein pollution
- High recovery – DNA can be recovered at the level of PG by silica gel column purification
Inhalt des Kits
| Inhalt | IVD3182 |
| Reinigungszeiten | 50 |
| Buffer ACL | 250 ml |
| Buffer ACB* | 300 ml |
| Buffer DCW1* | 22 ml |
| Buffer DCW2* | 10 ml |
| Proteinase K | 540 mg |
| Protease-Auflösungspuffer | 30 ml |
| Träger-RNA | 110 μg |
| Nuclease Free Water | 20 ml |
| HiPure CFDNA Mini Columns | 50 |
| 2 ml-Sammelröhrchen | 100 |
| Extender Tube | 50 |
| Vac-Connector | 50 |
Lagerung und Stabilität
- Proteinase K & RNA Carrier: Fridge (2-8°C) for best results.
- Other Kit Components: Zimmertemperatur (15-25°C) is good.
- Storing Cold? Warm buffers up before use.
Experimentdaten
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