HiPure Circulated DNA Midi Spin Kit D (Spin-Protokoll)

Einführung

HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:

• Targets:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Types:
  • Human plasma
  • Human serum
  • Human urine (fresh or frozen once)
• Focus on Short DNA: Captures short nucleic acids (<1000bp) commonly found in biofluids.
• Efficient Purification: Effectively recovers cfDNA from various biofluids.
• Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.

Vorteile:

• Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
• Breite Probenkompatibilität: Works with various human biofluids, offering flexibility in sample selection.
• Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsies, ultimately aiding in patient diagnosis and management.

Einzelheiten

Spezifikationen

Prinzip

This product utilizes silica gel columns for efficient DNA extraction:

1. Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
3. Waschen: Wash buffers to remove leftover proteins and cellular debris.
4. Elution: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

Vorteile

• Hohe Ausbeute – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration – low elution volume, ensuring high nucleic acid concentration
• Hohe Reinheit – low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery – DNA can be recovered at the level of PG by silica gel column purification

Inhalt des Kits

Lagerung und Stabilität

• Proteinase K & Träger-RNA: Fridge (2-8°C) for best results.
• Everything Else: Zimmertemperatur (15-25°C) is fine.
• Important: If storing cold, warm buffers before use.