Produktübersicht
- Offers fast and easy methods for purifying total DNA.
- Ensures reliable PCR and Southern blotting results.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Can purify DNA from small volumes of blood, tissue, and dry blood spots.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain |
| Anwendungen | PCR, Südlicher Bolzen und Virenerkennung, usw. |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Animal tissues, blood stain, urine, seminal stain and various forensic samples |
| Probenmenge | Blut:1-100μl, Gewebe:<10mg |
| Elutionsvolumen | |
| Zeit pro Lauf | |
| Flüssigkeitstransportvolumen pro Säule | |
| Bindungsausbeute der Säule |
Prinzip
- Utilizes silica column purification method.
- Sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nukleinsäure wird an der Membran adsorbiert, während Protein nicht adsorbiert wird und durch Filtration entfernt wird.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Vorteile
- Schnell – Es können mehrere Proben entnommen werden 20 Protokoll (after digestion)
- Hohe Reinheit – purified DNA can be directly used in various downstream applications
- High recovery – DNA can be recovered at the level of PG
- Good repeatability – silica technology can obtain ideal results every time
Inhalt des Kits
| Inhalt | D312502 | D312503 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| Puffer-ATL | 15 ml | 60 ml |
| Puffer AL | 15 ml | 60 ml |
| Buffer GW1* | 22 ml | 66 ml |
| Puffer GW2* | 20 ml | 2 X 50 ml |
| Träger-RNA | 310 μg | 2 X 310 µg |
| Proteinase K | 24 mg | 120 mg |
| Protease-Auflösungspuffer | 1.8 ml | 10 ml |
| Puffer AE | 15 ml | 60 ml |
| HiPure DNA Mini-Säulen I | 50 | 2 X 125 |
| 2 ml-Sammelröhrchen | 100 | 5 X 100 |
Lagerung und Stabilität
- Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival.
- Short-term storage at room temperature (15–25°C) for up to 12 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15–25°C) and remain stable for at least 18 Monate unter diesen Bedingungen.
Experimentdaten
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