This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 Protokoll. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection, and in vitro translation.
Einzelheiten
Inhalt des Kits
| Inhalt | R415002 | D415003 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| HiPure RNA Mini-Säulen | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2ml-Sammelröhrchen | 100 | 500 |
| TCEP (1M) | 0.29 G | 5 X 0.29 G |
| Buffer EP | 1.0 ml | 5.0 ml |
| Buffer PSL | 50 ml | 250 ml |
| Puffer RW1 | 50 ml | 250 ml |
| Puffer RW2* | 20 ml | 2 X 50 ml |
| RNase-freies Wasser | 10 ml | 30 ml |
Lagerung und Stabilität
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
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