Einführung

The HiPure Mini system provides a fast, simple, and cost-effective Plasmid-DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Einzelheiten

Spezifikationen

Prinzip

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. Nach dem Waschen von Proteinen und anderen Verunreinigungen, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9,0, 0.5mm EDTA).

Vorteile

• Hohe Reinheit – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, usw.
• Schnell – it takes only 2 minutes to obtain supernatant by optimized solution (10 minutes for other brands)
• Hohe Ausbeute – up to 70µg plasmid can be binded in one column

Inhalt des Kits

Lagerung und Stabilität

The kit components can be stored dry at room temperature (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen. Falls sich in den Puffern Ausfällungen bilden, Zum Auflösen auf 37 °C erwärmen. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Kaufratgeber