Einführung
- HiPure Plasmid-DNA Plus 96 Bausatz:
- Enables up to 96 mini-preps to be performed simultaneously.
- Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96).
- Provides a fast, simple, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications.
- Hauptmerkmale:
- Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries.
- Plasmid DNA purified with the kit is immediately ready for use.
- Eliminates the need for phenol extraction and ethanol precipitation.
- High-quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate |
| Anwendungen | Enzyme digestion, Sequenzierung, PCR, Beschriftung, usw. |
| Reinigungsmethode | 96 Brunnenplatte |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Conventional plasmid, plasmid less than 30KB |
| Probenmenge | 1-1.5ml（x96） |
| Ertrag | 1-15µg/1ml |
| Elutionsvolumen | ≥70μl |
| Zeit pro Lauf | ≤60 minutes |
| Flüssigkeitstransportvolumen pro Säule | 800µl |
| Bindungsausbeute der Säule | 70µg |
Prinzip
- HiPure Plasmid Procedure:
- Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
- Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps.
- Procedure Steps:
- Preparation and Clearing of Bacterial Lysate:
- The alkaline method is used to prepare and clear bacterial lysate.
- DNA Binding:
- Supernatant transferred to a column to bind DNA to the silica membrane.
- Washing and Elution:
- Proteins and other impurities washed away.
- Die Nukleinsäure wurde schließlich mit einem salzarmen Puffer eluiert (10mm Tris, pH9,0, 0.5mm EDTA).
- Preparation and Clearing of Bacterial Lysate:
Vorteile
- Hohe Reinheit – purified plasmid can be directly used in sequencing, enzyme digestion PCR, usw.
- Schnell – it takes only 60 minutes to complete the isolation
- Hohe Ausbeute – up to 1mg plasmid can be bound in one column
- Economy – high cost-performance
Inhalt des Kits
| Inhalt | P100601 | P100602 | P100603 |
| Reinigungszeiten | 1 X 96 Vorbereitungen | 4 X 96 Vorbereitungen | 20 X 96 Vorbereitungen |
| RNase A | 5 mg | 20 mg | 100 mg |
| Buffer P1 | 30 ml | 120 ml | 600 ml |
| Buffer P2 | 30 ml | 120 ml | 600 ml |
| Buffer P3 | 40 ml | 180 ml | 800 ml |
| Buffer PW1 | 100 ml | 500 ml | 2 X 1000 ml |
| Buffer PW2 | 50 ml | 2 X 100 ml | 4 X 200 ml |
| Elutionspuffer | 150 ml | 60 ml | 300 ml |
| Lysate Clear Plate | 1 | 4 | 20 |
| HiPure DNA-Platte | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.5ml Elute Plate | 1 | 4 | 20 |
Lagerung und Stabilität
The kit components can be stored dry at room temperature (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen. Falls sich in den Puffern Ausfällungen bilden, Zum Auflösen auf 37 °C erwärmen. After the addition of RNase A， Buffer P1 is stable for 6 months when stored at 2-8°C.
Kaufratgeber
| Name | KATZE NEIN | Probenmenge | Spaltentyp | Elutionsvolumen | Ertrag | Zeit pro Lauf | Centrifuge requirements |
| HiPure Plasmid Mini Kit | P1001 | 1-5ml | 1.5ml-Säule | 30-50μl | 5–35µg/5ml | ≤30 Minuten | small centrifuge |
| HiPure Plasmid Mini Kit II | P1002 | 5-15ml | 1.5ml-Säule | 75-100μl | 20-80µg/15ml | ≤30 Minuten | small centrifuge |
| HiPure Plasmid Plus 96 Bausatz | P1006 | 1-1.5ml | 96 Brunnenplatte | 70-150μl | 1-15μg/1ml | ≤60 minutes | 96-well plate centrifuge |
| HiPure Fastfilter Plasmid Midi Kit | P1013 | 30-50ml | 15ml-Säule | 0.3-0.8ml | 50–250µg/50ml | ≤60 minutes | 15ml centrifuge |
| HiPure Fastfilter Plasmid Maxi Kit | P1014 | 100-200ml | 50ml-Säule | 0.5-1.5ml | 0.4–1mg/200ml | ≤60 minutes | 50ml centrifuge |
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