Einführung
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total RNA and miRNA from 0.2-0.6ml serum and plasma |
| Anwendungen | |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | |
| Probenmenge | |
| Ertrag | |
| Elutionsvolumen | |
| Zeit pro Lauf | |
| Flüssigkeitstransportvolumen pro Säule | |
| Bindungsausbeute der Säule |
Prinzip
HiPure RNA technology simplifies total RNA isolation. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After the removal of protein the binding conditions for nucleic acids are adjusted by adding isopropanol. Total nucleic acids are bound to the column. Optionally, DNA can be removed by an on-column DNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-Free water.
Inhalt des Kits
| Inhalt | R431402 | R431403 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| HiPure RNA Mini-Säulen | 100 | 2 X 250 |
| 2ml-Sammelröhrchen | 100 | 2 X 250 |
| Buffer CFL | 6 ml | 30 ml |
| Buffer CFP | 1.8 ml | 10 ml |
| Buffer RWC | 20 ml | 80 ml |
| Puffer RW2* | 20 ml | 2 X 50 ml |
| RNase-freies Wasser | 10 ml | 60 ml |
Lagerung und Stabilität
The kit components can be stored at room temperature (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen.
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