- Challenges in DNA Extraction from Blood Samples:
- High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
- Complex DNA Source: Blood DNA contains various types such as mitochondrial, genomisch, and circulating DNA, leading to potential loss during extraction.
- Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
- Existing Extraction Methods:
- Phenol-chloroform extraction, salting out, usw.
- These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
- Product Benefits:
- Schnelle und einfache Reinigung: Provides a rapid and straightforward method for total DNA-Reinigung.
- Suitability: Purifies total DNA (genomisch, viral, mitochondrial) from whole blood, tissue, und kultivierte Zellen.
- Anwendung: Ensures reliable DNA for PCR and Southern blotting assays.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation of total DNA from 10ml blood and 1g tissue using Maxi column |
| Anwendungen | PCR, Südlicher Bolzen und Virenerkennung, usw |
| Reinigungsmethode | Maxi Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Gewebe, Zellen, Blut, Speichel, swabs, blood spots, semen, and other clinical samples |
| Probenmenge | 3-10ml |
| Elutionsvolumen | ≥700μl |
| Zeit pro Lauf | ≤90 minutes |
| Flüssigkeitstransportvolumen pro Säule | 4ml |
| Bindungsausbeute der Säule | 5mg |
Prinzip
- Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
- Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
- Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
- DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.
Vorteile
- Hochwertige DNA – erfüllt eine Vielzahl nachgelagerter Anwendungen, inklusive PCR, qPCR, Enzymverdauung, Hybridisierung, usw.
- Schnell – ohne Trennung von Leukozyten, organische Extraktion, oder Ethanolfällung
- Einfach – Alle Nukleinsäuren können durch Direktverdau gewonnen werden
- Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
- Breite Anwendbarkeit – Umgang mit einer Vielzahl flüssiger Proben
- Economy – excellent cost-effectiveness performance
Inhalt des Kits
| Contents10 | 10 Vorbereitungen | 50 perps |
| Reinigungszeiten | 10 | 50 |
| HiPure gDNA Maxi Columns | 10 | 50 |
| 50ml-Sammelröhrchen | 20 | 100 |
| Puffer-ATL | 120 ml | 550 ml |
| Puffer AL | 120 ml | 2 X 300 ml |
| Buffer GW1* | 53 ml | 220 ml |
| Puffer GW2* | 25 ml | 110 ml |
| RNase A | 40 mg | 180 ml |
| Proteinase K | 120 mg | 540 mg |
| Protease-Auflösungspuffer | 12 ml | 50 ml |
| Puffer AE | 30 ml | 120 ml |
Lagerung und Stabilität
Storage Recommendations:
- Proteinase K and RNase A: Upon arrival, store at 2-8°C. Short-term storage (bis zu 12 Wochen) bei Raumtemperatur (15-25°C) is acceptable without affecting performance.
- Remaining Kit Components: Can be stored at room temperature (15-25°C) and remain stable for at least 18 Monate unter diesen Bedingungen.
Kaufratgeber
| Name | KATZE NEIN | Probenmenge | Leukocyte protocol* | Colum type | Elutionsvolumen | Average yield | Zeit pro Lauf |
| HiPure Blood DNA Mini-Kit | D3111 | 10-200μl | 1ml | 2ml-Säule | ≥20μl | 5-9μg/200μl | ≤30 Minuten |
| HiPure-Gewebe&Blut-DNA-Midi-Kit | D3113 | 0.2-2ml | 10ml | 1.5ml-Säule | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure-Gewebe&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml-Säule | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure-Gewebe&Blut-DNA 96 Bausatz | D3117 | 1-200μl | 1ml | 96 Brunnenplatte | 3-8μg/200μl |
