Blood is a valuable source of DNA for clinical diagnostics and research, but traditional extraction methods present challenges:
- Safety Hazards: Bloodborne pathogens pose a risk of infection during extraction.
- DNA Loss: Red blood cell removal can eliminate desired DNA fractions (viral, zirkulierende DNA).
- Impurities and Inhibitors: Blood components can interfere with downstream applications (PCR, Southern Blot).
This product offers a novel DNA extraction solution that addresses these issues:
- Safe and Efficient: No red blood cell removal minimizes infectious waste and contamination risks.
- Comprehensive DNA Recovery: Purifies total DNA (genomisch, mitochondrial, viral, circulating) for a complete picture.
- Breite Probenkompatibilität: Works with whole blood (fresh/frozen), Plasma, Serum, Buffy-Mantel, und kultivierte Zellen.
- Downstream-Anwendungen: Purified DNA is suitable for PCR and Southern blotting.
This innovative method simplifies blood DNA extraction while ensuring safety, efficiency, and comprehensive DNA recovery. It represents a significant advancement for researchers in diagnostics, forensics, and medical research.
Notiz: This bulletin corrects the previous statement about sample compatibility. This method is not suitable for tissue samples.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| Anwendungen | PCR, Südlicher Bolzen und Virenerkennung, usw |
| Reinigungsmethode | Midi Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Gewebe, Zellen, Blut, Speichel, swabs, blood spot, semen, and other clinical samples |
| Probenmenge | 0.2-2 ml |
| Elutionsvolumen | ≥300μl |
| Zeit pro Lauf | ≤80 minutes |
| Flüssigkeitstransportvolumen pro Säule | 4ml |
| Bindungsausbeute der Säule | 1mg |
Principles
This product offers a streamlined method for purifying DNA from blood samples using silica column technology.
The Process:
- Lysis and Digestion: The blood sample is treated with a lysate solution and protease to break open cells and degrade proteins, releasing DNA into the solution.
- Binding to Silica: The lysate is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules, while proteins and other impurities pass through.
- Waschen: Wash buffers remove unbound proteins and cellular debris that didn’t adhere to the silica.
- Elution: A low-salt buffer (typically 10 mM Tris, pH 9.0, Und 0.5 mM EDTA) is used to elute the purified DNA from the silica. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.
Vorteile
- Hochwertige DNA – erfüllt eine Vielzahl nachgelagerter Anwendungen, inklusive PCR, qPCR, Enzymverdauung, Hybridisierung, usw.
- Schnell – ohne Trennung von Leukozyten, organische Extraktion, oder Ethanolfällung
- Einfach – Alle Nukleinsäuren können durch Direktverdau gewonnen werden
- Breite Anwendbarkeit – Umgang mit einer Vielzahl flüssiger Proben
Inhalt des Kits
| Inhalt | D311302 | D311303 |
| Reinigungszeiten | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15ml-Sammelröhrchen | 40 | 200 |
| Puffer-ATL | 50 ml | 250 ml |
| Puffer AL | 50 ml | 250 ml |
| Buffer GW1* | 22 ml | 110 ml |
| Puffer GW2* | 12 ml | 50 ml |
| RNase A | 20 mg | 90 mg |
| Proteinase K | 100 mg | 440 mg |
| Protease-Auflösungspuffer | 10 ml | 30 ml |
| Puffer AE | 20 ml | 120 ml |
Lagerung und Stabilität
For optimal performance:
- Store Proteinase K and RNase A at 2-8°C (refrigerator) upon arrival.
Acceptable short-term storage:
- Proteinase K and RNase A can be stored for up to 12 weeks at room temperature (15-25°C) without affecting their performance.
Remaining kit components:
- Store all other components of the kit dry at room temperature (15-25°C).
- They are stable for at least 18 Monate unter diesen Bedingungen.
Important Note:
- If storing the entire kit at room temperature, ensure buffers are redissolved before use and always warm all buffers to room temperature before using them.
Kaufratgeber
| Name | KATZE NEIN | Probenmenge | Leukocyte protocol* | Colum type | Elutionsvolumen | Average yield | Zeit pro Lauf |
| HiPure Blood DNA Mini-Kit | D3111 | 10-200μl | 1ml | 2ml-Säule | ≥20μl | 5-9μg/200μl | ≤30 Minuten |
| HiPure-Gewebe&Blut-DNA-Midi-Kit | D3113 | 0.2-2ml | 10ml | 1.5ml-Säule | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure-Gewebe&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml-Säule | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure-Gewebe&Blut-DNA 96 Bausatz | D3117 | 1-200μl | 1ml | 96 Brunnenplatte | 3-8μg/200μl |
