The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, Gewebe, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column |
| Anwendungen | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Animal soft tissue, cultured cells, Lymphozyten, simple plant tissue |
| Probenmenge | Cells: ≤1 x 107 Animal tissue: 1-20 mg Plant leaves: 50-150 mg Yeast cells: 5 X 106 |
| Ertrag | 2-100μg |
| Elutionsvolumen | ≥50μl |
| Zeit pro Lauf | ≤15 minutes（1-24 samples） |
| Flüssigkeitstransportvolumen pro Säule | 800µl |
| Bindungsausbeute der Säule | 100µg |
Prinzip
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Vorteile
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Gute Qualität – high purity total RNA can be directly used in various sensitive downstream applications
- Schnell – Es können mehrere Proben entnommen werden 20 minutes by column method
- Sicher – keine Phenol-Chloroform-Extraktion erforderlich
- Sensitive – RNA can be recovered at the level of PG
Inhalt des Kits
| Inhalt | R401102 | R401103 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| HiPure RNA Mini-Säulen | 50 | 250 |
| 2ml-Sammelröhrchen | 50 | 250 |
| RTL Lysis Buffer | 50 ml | 200 ml |
| RNA Binding Buffer | 15 ml | 75 ml |
| Puffer RW1 | 50 ml | 200 ml |
| Puffer RW2* | 20 ml | 2 X 50 ml |
| RNase-freies Wasser | 10 ml | 30 ml |
Lagerung und Stabilität
The Kit can be stored dry at room temperature (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen.
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