This product offers a convenient and rapid method to purify total DNA from a wide range of samples, making it ideal for various downstream analyses:
- Fast and Efficient: The protocol is streamlined for quick DNA extraction with minimal hands-on time.
- Hochwertige DNA: Das System isoliert die gesamte DNA (genomisch, viral, mitochondrial) mit hoher Reinheit, suitable for reliable PCR, quantitative PCR (qPCR), Southern Blot, and viral DNA detection.
- Breite Probenkompatibilität: The method effectively purifies DNA from a variety of sources, einschließlich:
- Gewebe
- Cells
- Blut
- Saliva
- Swabs
- Blood Spots
- Semen
- Other clinical samples
- Downstream-Anwendungen: The purified DNA can be used directly in various research techniques, einschließlich:
- PCR (Polymerase Kettenreaktion)
- qPCR (quantitative PCR)
- Southern Blotting
- Viral DNA Detection
- Other DNA-based analyses
This solution simplifies DNA extraction while maintaining high quality, making it a valuable tool for researchers working in various fields of molecular biology, forensics, and diagnostics.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| Anwendungen | PCR, qPCR, Südlicher Bolzen und Virenerkennung, usw |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Gewebe, Zellen, Blut, Speichel, swabs, blood spots, semen, and other clinical samples |
| Probenmenge | Solid tissue: 1-10mg; Antikoagulierendes Blut: 200μl |
| Elutionsvolumen | ≥20μl |
| Zeit pro Lauf | 30 – 60 Protokoll |
| Flüssigkeitstransportvolumen pro Säule | 800µl |
| Bindungsausbeute der Säule | 100µg |
Prinzip
- Purification Method: Utilizes silica column purification for efficient nucleic acid extraction.
- Sample Lysis and Digestion: Samples are lysed and digested using lysate and protease, facilitating the release of DNA into the lysate.
- Nucleic Acid Adsorption: The lysed sample is transferred to an adsorption column, where nucleic acids selectively adsorb onto the membrane.
- Protein Removal: Proteins, which do not adsorb to the membrane, are removed through filtration, ensuring purity of the extracted nucleic acid.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted nucleic acid.
- Elution: Nucleic acid is finally eluted from the membrane using a low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA), resulting in efficient recovery of high-quality nucleic acid.
Vorteile
- Hochwertige DNA – erfüllt eine Vielzahl nachgelagerter Anwendungen, inklusive PCR, qPCR, Enzymverdauung, Hybridisierung, usw.
- Schnell – ohne Trennung von Leukozyten, organische Extraktion, oder Ethanolfällung
- Einfach – Alle Nukleinsäuren können durch Direktverdau gewonnen werden
- Breite Anwendbarkeit- Umgang mit einer Vielzahl flüssiger Proben
Inhalt des Kits
| Inhalt | D301802 | D301803 |
| Reinigungszeiten | 50 | 250 |
| HiPure DNA Mini-Säulen I | 50 | 250 |
| 2ml-Sammelröhrchen | 100 | 500 |
| Puffer-ATL | 30 ml | 150 ml |
| Puffer AL | 30 ml | 150 ml |
| Buffer GW1 | 22 ml | 88 ml |
| Puffer GW2 | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Protease-Auflösungspuffer | 1.8 ml | 10 ml |
| Puffer AE | 15 ml | 60 ml |
Lagerung und Stabilität
- Proteinase K Storage: Upon arrival, store Proteinase K at 2-8°C. Short-term storage (bis zu 12 Wochen) bei Raumtemperatur (15-25°C) is acceptable without affecting performance.
- Remaining Kit Components Storage: Die restlichen Kit-Komponenten können bei Raumtemperatur gelagert werden (15-25°C) and remain stable for at least 18 Monate unter diesen Bedingungen.
- Whole Kit Storage: The entire kit can also be stored at 2-8°C. Jedoch, in this case, buffers should be redissolved before use. Ensure all buffers are at room temperature when used for optimal performance.
Experimentdaten
