Dieses Produkt bietet schnelle und einfache Methoden zur zuverlässigen Reinigung der Gesamt-DNA PCR and southern blotting. Gesamt-DNA (z.B., genomisch, plasmid) can be purified from bacterial cultures.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total DNA from bacterial culture |
| Anwendungen | PCR, southern blot and enzyme digestion, usw. |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Manuell oder automatisch |
| Beispielstyp | Gram negative or positive bacteria |
| Probenmenge | Bacterial culture: 0.5-1.5ml |
| Elutionsvolumen | ≥60μl |
| Zeit pro Lauf | ≤60 minutes |
Prinzip
- This product utilizes high-binding magnetic particles for purification.
- Samples are lysed and digested using a lysate and Lysozyme.
- DNA is released into the lysate during this process.
- Magnetic particles and binding solution are added, allowing DNA to be adsorbed onto the surface of the particles while impurities such as proteins are not adsorbed.
- The adsorbed particles undergo washing with a washing solution to remove proteins and impurities.
- Ethanol is used for further washing to remove salts.
- Finally, DNA is eluted using Elution Buffer.
Vorteile
- Schnell: Multiple samples can be extracted within 60 Protokoll (after digestion), making the process time-efficient.
- Hohe Reinheit: Unique adsorbents ensure the complete removal of inhibitory factors, resulting in high-purity DNA extraction.
- High recovery: DNA can be recovered at the level of picograms (PG), indicating a high level of efficiency in the extraction process.
Inhalt des Kits
| Inhalt | D513101 | D513102 | D513103 |
| Reinigungszeiten | 48 Vorbereitungen | 96 Vorbereitungen | 480 Vorbereitungen |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease-Auflösungspuffer | 3 ml | 6 ml | 30 ml |
| Puffer Sicherheitsdatenblatt | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Buffer GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Lagerung und Stabilität
- MagPure Particles, Lysozyme, and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage (bis zu 12 Wochen) bei Raumtemperatur (15-25°C) hat keinen Einfluss auf ihre Leistung.
- Die restlichen Kit-Komponenten können trocken bei Raumtemperatur gelagert werden (15-25°C) and remain stable for at least 18 Monate unter diesen Bedingungen.
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