This product supplies a simple and rapid extraction of total RNA from Blood, Buffy-Mantel, Knochenmark, cell suspension, and other body fluids. The kit is based on superparamagnetic particle purification technology, with no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing, and so on.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total RNA from 300μl whole blood, Knochenmark, Buffy-Mantel |
| Anwendungen | RT-PCR, cDNA-Synthese, second-generation sequencing |
| Reinigungsmethode | Polydisperse Magnetperlen |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Manuell oder automatisch |
| Beispielstyp | Vollblut, Buffy-Mantel, Knochenmark, cultured cells, Lymphozyten, body fluid |
| Probenmenge | ≤5×106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow |
| Ertrag | 1-30μg |
Prinzip
The Kit can be used for both manual extraction processes and automatic nucleic acid extraction machines. This Kit is suitable for extracting RNA from ≤5×106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow. This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. RNA/DNA is released into the lysate. Nach Zugabe von Magnetpartikeln und Bindelösung, DNA/RNA wird auf der Oberfläche magnetischer Partikel adsorbiert, und Verunreinigungen wie Proteine werden ohne Adsorption entfernt. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, mit Ethanol gewaschen, um Salze zu entfernen, and finally, RNA was eluted by Elution Buffer.
Vorteile
- Hohe Reinheit – OD 260 / 280:1.9-2.0, OD 260 / 230:1.5-2.0
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – extraction without manual participation, saving time and effort
- Hohe Ausbeute – nucleic acid recovery up to 95%
- Allgemein – samples include whole blood, Serum, Plasma, lymphocyte suspension, Knochenmark, cultured cells, usw.
Inhalt des Kits
| Inhalt | R661101 | R661102 | R661103 |
| Reinigungszeiten | 48 Vorbereitungen | 96 Vorbereitungen | 480 Vorbereitungen |
| MagPure RNA Particles | 1.7 ml | 3.5 ml | 18 ml |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease-Auflösungspuffer | 1.8 ml | 5 ml | 15 ml |
| DNase I | 600 μl | 2 X 600 μl | 10 X 600 µl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Buffer MLB | 40 ml | 80 ml | 350 ml |
| Buffer MCB* | 15 ml | 30 ml | 150 ml |
| Buffer MW1* | 22 ml | 44 ml | 220 ml |
| Buffer MW2* | 20 ml | 50 ml | 2 X 100 ml |
| RNase-freies Wasser | 10 ml | 15 ml | 120 ml |
Lagerung und Stabilität
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Jedoch, kurzfristige Lagerung (DNase I up to 1 week, MagPure RNA Particles and Proteinase K up to 8 Wochen) bei Raumtemperatur (15–25°C) hat keinen Einfluss auf ihre Leistung. Die restlichen Kit-Komponenten können bei Raumtemperatur gelagert werden (15–25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen.
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