Einführung
This kit empowers researchers with a streamlined solution for isolating high-quality circulating cell-free DNA (cfDNA) from various biofluids:
- Sample Types: Plasma, Serum (1-6ml)
- Target: cfDNA molecules
- Downstream-Anwendungen:
- PCR (polymerase chain reaction)
- Real-time PCR
- Biochip analysis
- Sequenzierung der nächsten Generation (NGS)
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation circulating DNA from 1-6ml plasma, Serum, body fluids |
| Anwendungen | qPCR, NGS, usw. |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Manuell oder automatisch |
| Beispielstyp | Serum, Plasma |
| Probenmenge | 1-6ml |
| Elutionsvolumen | ≥50μl |
| Zeit pro Lauf | ≤60 minutes |
Prinzip
This product utilizes innovative magnetic bead technology for efficient DNA extraction:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
- Binding to Magnetic Beads: Magnetic particles coated for high DNA affinity are added to the lysate. DNA molecules selectively bind to the surface of these beads, while impurities like proteins remain unbound.
- Washing and Purification: A series of washes with different solutions remove unbound proteins and other cellular debris.
- Elution: A special elution buffer disrupts the interaction between the DNA and the magnetic beads, allowing the purified DNA to be collected in a separate tube.
Vorteile
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – without labor
- Hohe Reinheit – OD 260 / 280: 1.7-1.9, OD 260 / 230:1.5-2.0
Inhalt des Kits
| Inhalt | IVD5435 |
| Reinigungszeiten | 50 |
| MagPure Particles F | 14 ml |
| Träger-RNA | 310 μg |
| Proteinase K | 240 mg |
| Protease-Auflösungspuffer | 15 ml |
| Puffer Sicherheitsdatenblatt | 15 ml |
| Buffer MLK | 500 ml |
| Buffer MAW1 | 250 ml |
| Buffer MW2* | 50 ml |
| Elutionspuffer | 60 ml |
Lagerung und Stabilität
Ideal Storage:
- Store Proteinase K, Träger-RNA, and MagPure Particles F in the refrigerator (2-8°C) upon arrival for maximum effectiveness.
Acceptable Short-Term Storage:
- These components can be kept at room temperature (15-25°C) for up to 12 weeks without impacting their performance.
Storing Remaining Components:
- All other kit components can be stored dry at room temperature (15-25°C).
- They will remain stable for at least 18 Monate unter diesen Bedingungen.
Important Notes:
- If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
- Always ensure all buffers reach room temperature before using them for optimal results.
Experimentdaten
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