This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 Protokoll. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total RNA from tissue and cells and plant |
| Anwendungen | RT-PCR, cDNA-Synthese, second generation sequencing |
| Reinigungsmethode | Polydisperse Magnetperlen |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Manuell oder automatisch |
| Adaptives Instrument | Nucleic acid extractor, pipetting workstation |
| Beispielstyp | Animal tissue, human tissue, cultured cells, Lymphozyten, ordinary plant tissue |
| Probenmenge | Cells: 1 X 107 Animal tissue: ≤20mg Plant tissue: ≤100mg Yeast cell: ≤5 x 106 |
| Ertrag | 5-100μg |
Prinzip
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Vorteile
- Hohe Reinheit – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – extraction without manual participation, saving time and effort
- Universal – suitable for various animal tissues, cultured cells and conventional plant fungal tissues
Inhalt des Kits
| Inhalt | R662201 | R662202 | R662203 |
| Reinigungszeiten | 48 Vorbereitungen | 96 Vorbereitungen | 5 X 96 Vorbereitungen |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| Proteinase K | 24 mg | 50 mg | 240 mg |
| Protease-Auflösungspuffer | 1.8 ml | 5 ml | 15 ml |
| DNase I | 600 μl | 2 X 600 μl | 10 X 600 μl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| RTL Lysis Buffer | 40 ml | 80 ml | 400 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 X 220 ml |
| Puffer RW2* | 20 ml | 50 ml | 2 X 100 ml |
| RNase-freies Wasser | 10 ml | 30 ml | 120 ml |
Lagerung und Stabilität
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. Jedoch, kurzfristige Lagerung (DNase I up to 1 Wochen, MagPure RNA Particles and Proteinase K up to 8 Wochen) bei Raumtemperatur (15–25°C) hat keinen Einfluss auf ihre Leistung. Die restlichen Kit-Komponenten können bei Raumtemperatur gelagert werden (15–25°C) and are stable for atleast 18 Monate unter diesen Bedingungen.
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