This product speeds and simplifies nucleic acid size selection for fragment library preparation for next generation sequencing.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Selectively recover DNA from PCR products and enzymatic reaction solution （Replace Beckmen or agencourt SPRISelect） |
| Anwendungen | Prepare DNA library for second generation sequencing |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Manuell oder automatisch |
| Beispielstyp | DNA-Produkte, restriction endonuclease systems, or other enzymatic reaction solution |
| Probenmenge | Appropriate |
| Erholung | 80% |
| Operation time | ≤50 minutes |
Prinzip
The MagSelect method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger to paramagnetic beads. Excessprimers, nucleotides, salts, and enzymes can be removed using a simple washing procedure. The result is a more purified PCR product.
Vorteile
- High recovery – bis zu 80%
- High throughput – using magnetic beads purification technology
Inhalt des Kits
| Inhalt | XP-5 | XP-50 | XP-500 |
| MagSelect Beads | 5 ml | 50 ml | 500 ml |
Lagerung und Stabilität
MagSelect XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. Jedoch, kurzfristige Lagerung (bis zu 12 Wochen) bei Raumtemperatur (15-25°C) hat keinen Einfluss auf seine Leistung. Shake the reagent well before use. It should appear homogenous and consistent in color.
DO NOT FREEZE.
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