Einführung
- RNA Clean Beads:
- Utilize paramagnetic bead technology for high-throughput purification of RNA or cDNA.
- Designed for in vitro applications such as transcription, antisense RNA (aRNA) Verstärkung, and RNA and cDNA probe synthesis.
- Anwendungen:
- The resulting purified product can be used in various applications, einschließlich:
- PCR and RT-PCR
- Probes for microarray or macroarray
- RNase protection assays
- Transfection for RNAi experiments
- cDNA-Synthese and labeling
- The resulting purified product can be used in various applications, einschließlich:
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Recovery of RNA from RNA reaction solution by magnetic bead method (Replace Beckmen or agencourt AmPure XP) |
| Anwendungen | Advanced applications, such as sequencing, genechip, fluorescence quantification, usw. |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Manuell oder automatisch |
| Beispielstyp | RNA products, restriction endonuclease systems, or other enzymatic reaction solutions |
| Probenmenge | 20-100µl |
| Erholung | 90% |
| Elutionsvolumen | ≥20μl |
| Operation time | ≤50 Protokoll |
Prinzip
RNA Clean Process:
- Selektive Bindung:
- Utilizes an optimized buffer to selectively bind RNA or cDNA to paramagnetic beads.
- Contaminant Removal:
- Excess oligonucleotides, nucleotides, salts, and enzymes are removed through a simple washing procedure.
Vorteile
- High recovery – recovery of RNA up to 90%
- High throughput – using magnetic beads purification technology
- Einfach – simplified operation process, combination – washing – elution
Inhalt des Kits
| Inhalt | BRP-5 | BRP-50 | BRP-500 |
| RNA Clean Beads | 5 ml | 50 ml | 500 ml |
Lagerung und Stabilität
DO NOT FREEZE.
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