Einführung
- Produktqualität: Subject to stringent screening and quality control measures, the product serves as the fundamental material for nucleic acid extraction, ensuring reliability and efficiency.
- Triton X-100 Properties: Triton X-100 is a non-ionic surfactant featuring both hydrophilic and hydrophobic ends. Its stability in aqueous solutions, resistance to strong electrolytes, and ability to form soluble complexes with lipids, such as phospholipids, contribute to its versatile functionality.
- Membrane Interaction: The hydrophobic end of Triton X-100 interacts with the hydrophobic regions of membrane proteins, facilitating their dissolution in solution. This property aids in increasing cell membrane permeability and disrupting cell membranes, enhancing nucleic acid release.
- Lipid Dissolution: Triton X-100 can dissolve lipids, further augmenting cell membrane permeability and promoting cell membrane rupture.
- Synergy with Sicherheitsdatenblatt: When combined with SDS, Triton X-100 synergistically enhances cell lysis and disrupts the surface membrane structure of enveloped viruses. This synergy facilitates efficient nucleic acid release from cells.
- Antagonistic Effect: Triton X-100 counteracts the residual effects of SDS, inhibiting any adverse impact on subsequent processes. This antagonistic property ensures the smooth progression of nucleic acid extraction procedures.
Spezifikationen
| Merkmale | Spezifikation | |
| Grundlegende Inhalte | Empfohlene Anwendung | DNA / RNA-Extraktion, Extraktion viraler Nukleinsäuren |
| CAS-Nummern | 9002-93-1 | |
| Formel | C18H30O3 | |
| Ebene | Molekularbiologie | |
| Aussehen | White transparent or translucent liquid | |
| Transportbedingungen | Zimmertemperatur | |
| Aufbewahrungsbedingungen | Zimmertemperatur | |
| Quality guarantee period | 2 Jahre | |
| Verunreinigungsparameter | Geruch | Nothing |
| Säurezahl (mgKOH/g) | ≤1.0% | |
| Verseifungswert (mgKOH/g) | 45-60 | |
| Relative viscosity | 1.2-1.5 | |
| Feuchtigkeit | ≤1.0% | |
| Iron | ≤5ppm | |
| Potassium | ≤0.05% | |
| Heavymetal ion | ≤5ppm | |
| UV-Absorptionswert | Absorptionswert @ 230 (1%) | ≤30 |
| Absorptionswert @ 260 (1%) | ≤15 | |
| Absorptionswert @ 280 (1%) | ≤30 | |
| Absorptionswert @ 320 (1%) | ≤0.1 | |
| Im Zusammenhang mit der Extraktion von Nukleinsäuren | DNA-Extraktionstest | Adoptieren |
| Virus-Gesamtnukleinsäuretest | Adoptieren | |
| DNase-Test (5%) | Nicht erkannt | |
| RNase-Test (5%) | Nicht erkannt | |
| Impurity analysis (100%) | 60 degrees full solution |
Bestellinformationen
| Katze nein. | Produktname | Paket |
| C11209 | Triton X-100, for molecular biology | 4000 ml |
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