Introducción
- Tecnología: Utilizes paramagnetic bead technology for rapid and high-performance dye-terminator removal.
- No Centrifugation or Filtration: Eliminates the need for centrifugation or filtration steps, simplifying the purification process.
- Manual or Automated: This can be performed manually or fully automated for high-throughput applications, providing flexibility in workflow.
- Improved Sequencing Results: Produces sequences with longer Phred 20 read lengths and higher signal intensities compared to similar purification systems.
- Enhanced Performance: Offers superior performance in terms of sequence quality and signal intensity, enhancing downstream analysis and interpretation.
Especificaciones
| Características | Especificaciones |
| Funciones principales | Eliminación del tinte fluorescente libre de la solución de secuenciación. (Reemplazar Beckmen o Agincourt CleanSeq) |
| Aplicaciones | Secuencias automatizadas |
| Tecnología de purificación | Tecnología de cuentas magnéticas |
| método de proceso | Manual o automático |
| Tipo de ejemplo | ADN dopado con tinte fluorescente |
| Cantidad de muestra | 5µl |
| Recuperación | 90% |
| Volumen de elución | ≥25μl |
| Tiempo de operacion | ≤50 minutos |
Principio
CleanSeq offers a rapid and efficient method for purifying sequencing reaction products, ideal for applications like Sanger sequencing.
- Magnetic Bead Technology: Utilizes magnetic particles within an optimized binding buffer to selectively capture desired DNA fragments.
- In-Plate Purification: The entire protocol can be performed directly in the thermal cycling plate, eliminating the need for transfer steps.
- Efficient Cleanup: Removes unincorporated dyes, nucleótidos, salts, and contaminants through a simple washing procedure.
- Automation Friendly: No centrifugation or filtration is required, making CleanSeq compatible with various automated liquid handling platforms.
This streamlined approach minimizes processing time and simplifies workflow, making CleanSeq a valuable tool for high-throughput sequencing applications.
Ventajas
- Alta recuperación – hasta 90%
- Alto rendimiento – utilizando tecnología de purificación de perlas magnéticas
- Simple – proceso de operación simplificado, combinación – Lavado – elución
Contenido del kit
| Contenido | BCS-5 | BCS-50 | BCS-500 |
| Perlas CleanSeq | 5 ml | 50 ml | 500 ml |
Almacenamiento y estabilidad
Almacenamiento:
- For optimal performance, store CleanSeq Beads at 2-8°C (refrigerator) upon arrival and up to 6 meses.
- Short-term storage (hasta 12 semanas) a temperatura ambiente (15-25°C) is acceptable without affecting performance.
Before Use:
- Ensure proper mixing of CleanSeq Beads before each use.
- The reagent should appear homogenous and consistent in color throughout.
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