Introducción
HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:
- Objetivos:
- Tumor-derived cfDNA fragments and mRNAs in blood
- Fetal nucleic acids in maternal blood
- Sample Types:
- Human plasma
- Human serum
- Human urine (fresh or frozen once)
- Focus on Short DNA: Captures short nucleic acids (<1000pb) commonly found in biofluids.
- Efficient Purification: Effectively recovers cfDNA from various biofluids.
- Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.
Beneficios:
- Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
- Broad Sample Compatibility: Works with various human biofluids, offering flexibility in sample selection.
- Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
- Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.
This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsies, ultimately aiding in patient diagnosis and management.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Aislamiento de ADN circulante a partir de 1-5 ml de plasma., suero, body fluids by spin |
| Aplicaciones | qPCR, liquid or solid chip analysis, hibridación, and SNP detection, etc.. |
| Método de purificación | Mini columna de giro |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugation) |
| Tipo de ejemplo | Suero, plasma, and other cell-free fluid samples |
| Cantidad de muestra | 1-5ml |
| Volumen de elución | ≥50μl |
| Tiempo por carrera | ≤70 minutos |
| Volumen de transporte de líquido por columna | 4ml |
| Rendimiento de unión de la columna | 1mg |
Principio
This product utilizes silica gel columns for efficient DNA extraction:
- Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
- Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
- Washing: Wash buffers to remove leftover proteins and cellular debris.
- Elution: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Ventajas
- Alto rendimiento – proceso más óptimo, free DNA (>50pb) can be obtained to the maximum extent
- High concentration – low elution volume, ensuring high nucleic acid concentration
- Alta pureza – low alcohol binding method, completely removing inhibitor and protein pollution
- Alta recuperación – DNA can be recovered at the level of PG by silica gel column purification
Contenido del kit
| Contenido | D318203D |
| Tiempos de purificación | 50 preparativos |
| Buffer ACL | 250 ml |
| Buffer ACB* | 300 ml |
| Buffer DCW1* | 22 ml |
| Buffer DCW2* | 10 ml |
| Proteinasa K | 540 mg |
| Tampón de disolución de proteasa | 30 ml |
| Carrier RNA | 110 µg |
| Agua libre de nucleasas | 20 ml |
| HiPure CFDNA Mini Columns | 50 |
| 2 Tubos de recolección de ml | 100 |
| Extender Tubes | 50 |
| Sealing O-Ringes | 50 |
| 50ml Collection Tube | 50 |
| Support Tube | 50 |
Almacenamiento y estabilidad
- Proteinasa K & Carrier RNA: Fridge (2-8°C) for best results.
- Everything Else: Temperatura ambiente (15-25°C) is fine.
- Important: If storing cold, warm buffers before use.
Reseñas
Aún no hay reseñas.