Introducción
The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidina-based sample lysis and silica-membrane purification of total RNA. Reactivo MagZol LS, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini columna de giro.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation of total RNA from 0.25ml body fluids using column and MagZol LS reagent |
| Aplicaciones | qPCR / RT-PCR, liquid or solid-phase chip analysis, hibridación, and SNP detection |
| Método de purificación | Mini columna de centrifugado |
| Tecnología de purificación | tecnología de sílice, acid phenol/guanidine extraction technology (MagZol pretreatment technology) |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Sangre, suero, plasma |
| Cantidad de muestra | 250µl |
| Volumen de elución | ≥20µl |
| Volumen de transporte de líquido por columna | 800l |
| Rendimiento de unión de la columna | 100µg |
Principio
0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (hasta 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.
Ventajas
- Alta calidad – El ARN total de alta pureza se puede utilizar directamente en diversas aplicaciones sensibles posteriores.
- Fast – isolation of several samples can be completed in 40 minutes by using the column purification method
- Seguridad – no phenol-chloroform extraction is required
- Sensible – direct lysis of blood, plasma, and other samples without separation of leukocytes
Contenido del kit
| Contenido | R416302 | R416303 |
| Tiempos de purificación | 50 preparativos | 250 preparativos |
| Minicolumnas de ARN HiPure | 50 | 250 |
| 2Tubos de recolección de ml | 50 | 2 X 125 |
| Reactivo MagZol LS | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 60 ml |
| Búfer RW2* | 20 ml | 2 X 50 ml |
| Agua libre de ARNasa | 10 ml | 30 ml |
Almacenamiento y estabilidad
MagZol LS Reagent should be stored at 2-8°C upon arrival. Sin embargo, almacenamiento a corto plazo (hasta 24 semanas) a temperatura ambiente (15-25°C) no afecta su rendimiento. Los componentes restantes del kit se pueden almacenar secos a temperatura ambiente. (15-25°C) y son estables durante al menos 18 meses en estas condiciones.
Reseñas
Aún no hay reseñas.