This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 minutos. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, protección del ácido nucleico, y traducción in vitro.
Detalles
Contenido del kit
| Contenido | R415002 | D415003 |
| Tiempos de purificación | 50 preparativos | 250 preparativos |
| Minicolumnas de ARN HiPure | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2Tubos de recolección de ml | 100 | 500 |
| TCEP (1METRO) | 0.29 gramo | 5 X 0.29 gramo |
| Buffer EP | 1.0 ml | 5.0 ml |
| Buffer PSL | 50 ml | 250 ml |
| Memoria intermedia RW1 | 50 ml | 250 ml |
| Búfer RW2* | 20 ml | 2 X 50 ml |
| Agua libre de ARNasa | 10 ml | 30 ml |
Almacenamiento y estabilidad
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) y son estables durante al menos 18 meses en estas condiciones. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
Reseñas
Aún no hay reseñas.