Introducción
HiPure Plasmid EF Maxi Kit Features:
- Technology Integration:
- The kit integrates HiPure technology for plasmid purification with Magen’s innovative Endotoxin Removal Technology (ETR).
- Endotoxin Removal:
- Specifically designed to achieve low levels of endotoxins in the purified ADN plásmido, enhancing its suitability for sensitive applications.
- Application Suitability:
- Ideal for use in eukaryotic transfection and various in vitro experiments due to the high purity and low endotoxin levels of the DNA.
- Capacity and Yield:
- Capable of purifying up to 1000 µg of high copy number plasmid DNA or 200 µg of low copy number plasmid DNA.
- Culture Volume Processing:
- Efficient purification from up to 150 mL of an overnight bacterial culture.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation of up to 1.5 mg endotoxin-free plasmid DNA from 200ml bacterial culture |
| Aplicaciones | Transfección celular, inyección de animales, etc.. |
| Método de purificación | Maxi columna de giro |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Plásmido convencional, large plasmid |
| Cantidad de muestra | High copy plasmid: 100-150ml culture medium Low copy plasmid: 150-200ml culture medium |
| Producir | 100-1500µg |
| Volumen de elución | ≥0,8 ml |
| Tiempo por carrera | ≤60 minutos |
| Volumen de transporte de líquido por columna | 20ml |
| Rendimiento de unión de la columna | 1 mg |
Principio
HiPure Plasmid Procedure:
- Alkaline Lysis and DNA Adsorption:
- Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
- The unique silica membrane in the kit replaces glass or silica slurries for plasmid DNA minipreps.
- Three Basic Steps:
- Step 1: Preparation and clearing of a bacterial lysate using the alkaline method.
- Step 2: Transfer of the supernatant to a column for DNA binding.
- Step 3: Washing to remove proteins and other impurities, followed by elution of nucleic acid with low-salt buffer (10 mm tris, pH 9.0, 0.5 mmEDTA).
Ventajas
- High purity — purified plasmid can be directly used in sequencing, digestión enzimática y PCR, etc..
- Fast — silica gel column purification is much faster than ion exchange
- High yield — up to 1.5 mg plasmid can be binded in one column
- Ultra low endotoxin — content of endotoxin is less than 0.05 EU/μg, which can be directly used for cell transfection and animal injection, etc..
Contenido del kit
| Contenido | P115602 | P115603 |
| Tiempos de purificación | 10 preparativos | 50 preparativos |
| ARNasa A | 30 mg | 150 mg |
| Búfer P1 | 100 ml | 500 ml |
| Buffer P2 | 100 ml | 500 ml |
| Buffer LN3 | 50 ml | 250 ml |
| Buffer PW1 | 33 ml | 180 ml |
| Buffer PW2 | 20 ml | 100 ml |
| Tampón de elución | 15 ml | 120 ml |
| Buffer CL | 33 ml | 180 ml |
| HiPure DNA Maxi Columns C | 10 | 50 |
| Jeringa midi transparente lisado | 10 | 50 |
| 50 Tubos de recolección de ml, C | 20 | 100 |
Almacenamiento y estabilidad
Storage Instructions:
- General Storage:
- Kit components can be stored dry at room temperature (15–25 °C).
- Stable for at least 18 meses en estas condiciones.
- Buffer Precipitates:
- Si se forma algún precipitado en los tampones, warm at 37°C to dissolve.
- Specific Storage for RNase A and Buffer P1:
- RNase A and Buffer P1 are stable for 6 months when stored at 2–8 °C.
Guía de compra
| Nombre | NO GATO | Cantidad de muestra | Tipo de columna | Volumen de elución | Producir | Tiempo por carrera | Requisitos de centrífuga |
| Mini kit HiPure Plasmid EF | P1154 | 10-15ml | 1.5columna de ml | ≥75μl | 10–70 µg/15 ml | ≤40 minutos | pequeña centrífuga |
| Plásmido HiPure EF 96 Equipo | P1157 | 1-5ml | 96 buen plato | ≥75μl | 30µg/1ml | ≤60 minutos | 96 centrífuga de placa de pocillos |
| Kit midi HiPure Plasmid EF | P1231 | 30-50ml | 15columna de ml | ≥0,4 ml | 10-500microgramos/50ml | ≤60 minutos | 15centrífuga ml |
| Kit maxi HiPure Plasmid EF | P1156 | 100-200ml | 50columna de ml | ≥0,8 ml | 100–1500 µg/200 ml | ≤60 minutos | 50centrífuga ml |
| Megakit HiPure Plasmid EF | P1116 | 500ml | 50columna de ml | ≥0,8 ml | 10mg/500ml | ≤70 minutos | 50centrífuga ml |
Reseñas
Aún no hay reseñas.