Introducción
HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purificación, nucleic acid protection and in vitro translation.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Aislamiento de ARN total de 500 mg soil sample |
| Aplicaciones | RT-PCR, Northern blot, poly-A + purificación, nucleic acid protection and in vitro translation |
| Método de purificación | Mini columna de giro |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Forest soil, grassland soil, mining area soil, sediment and other samples |
| Cantidad de muestra | 500 mg |
| Volumen de elución | ≥30μl |
| Tiempo por carrera | ≤60 minutos |
| Volumen de transporte de líquido por columna | 800l |
| Rendimiento de unión de la columna | 100µg |
Principio
The hipure silica gel column is based on a glass fiber filter membrane with a high binding force. Under the condition of a high concentration of ionizing agent (como guanidina hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonds and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Ventajas
- Alta calidad – El ARN total de alta pureza se puede utilizar directamente en diversas aplicaciones sensibles posteriores.
- Rápido – extraction of several samples can be completed in 60 minutes by the column purification method
- Seguro – no phenol-chloroform extraction required
- Sensible – RNA can be purified at the level of PG
Contenido del kit
| Contenido | R418302 | R418303 |
| Tiempos de purificación | 50 preparativos | 250 preparativos |
| HiPure RNA Micro Columns | 50 | 250 |
| 2Tubos de recolección de ml | 100 | 500 |
| gDNA Filter Column | 50 | 250 |
| 2ml Beads Tubes | 50 | 250 |
| Buffer SOL | 30 ml | 150 ml |
| Buffer Ficha de datos de seguridad (MSDS) | 4 ml | 15 ml |
| Buffer PHC | 30 ml | 150 ml |
| Buffer GDP | 40 ml | 150 ml |
| Memoria intermedia RW1 | 50 ml | 200 ml |
| Buffer RW2 * | 20 ml | 2 X 50 ml |
| Agua libre de ARNasa | 10 ml | 30 ml |
Almacenamiento y estabilidad
Buffer PHC should be stored at 2–8°C upon arrival. Sin embargo, almacenamiento a corto plazo (hasta 12 semanas) a temperatura ambiente (15–25°C) no afecta su rendimiento. Los componentes restantes del kit se pueden almacenar secos a temperatura ambiente. (15–25°C) y son estables durante al menos 18 meses en estas condiciones. The entire kit can be stored at 2–8°C, but in this case, buffers should be redissolved before use. Asegúrese de que todos los tampones estén a temperatura ambiente cuando se utilicen..
Datos del experimento
Reseñas
Aún no hay reseñas.