Introducción
- Objetivo: Extraction of stool RNA.
- Sample Compatibility: Suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples.
- Tecnología: Utilizes silica gel column purification technology and an original solution system.
- Key Feature: Effectively removes humic acid and other inhibitory factors present in stool samples.
- Aplicaciones: The purified RNA can be directly used in RT-PCR, hibridación del norte, y otros experimentos.
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation total RNA from 100-150mg stool sample |
| Aplicaciones | RT-PCR, hibridación del norte, y otros experimentos |
| Método de purificación | Mini columna de giro |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Heces |
| Cantidad de muestra | 100-150 mg |
| Volumen de elución | ≥30μl |
| Tiempo por carrera | ≤50 minutos |
| Volumen de transporte de líquido por columna | 100µg |
| Rendimiento de unión de la columna | 800l |
Principio
- Principle of HiPure Silica Gel Column: Utilizes a high binding ability glass fiber filter membrane as the substrate. Under high concentrations of ionizing agents (p.ej., Guanidinium chloride or guanidina isotiocianato), nucleic acids are adsorbed through hydrogen bonding and electrostatic interactions, while proteins and other impurities are not adsorbed and removed. Subsequently, the filter membrane with adsorbed nucleic acids is washed to remove proteins and salts. Finally, low salt buffer solution (p.ej., Buffer TE) or water is used to elute the purified nucleic acids, resulting in high purity suitable for downstream experiments.
- Procesamiento de muestras: Stool samples are homogenized in the lysis solution, then further lysed in a high-temperature water bath to release RNA into the solution. Chloroform extraction is employed to remove genomic DNA and impurities. The supernatant is transferred to an alcohol-free binding solution, followed by RNA purification through a column. Finally, RNA is eluted using RNase Free Water.
- Aplicaciones: The purified RNA is suitable for various downstream experiments such as PCR, Southern hybridization, and enzyme digestion.
Ventajas
- Alta pureza: Utilizes a unique adsorbent for more efficient removal of inhibitors, ensuring high purity of extracted RNA.
- High Concentration: Achieves maximum extraction of total RNA from stool samples due to its high concentration, maximizing RNA yield.
- Sensible: Capable of purifying RNA at the level of picograms (PG), ensuring sensitivity in downstream applications.
Contenido del kit
| Contenido | R418502 | R418503 |
| Tiempos de purificación | 50 preparativos | 250 preparativos |
| Minicolumnas de ARN HiPure | 50 | 250 |
| 2Tubos de recolección de ml | 50 | 250 |
| Cuentas de vidrio (0.1~0.6mm) | 30 gramo | 150 gramo |
| Buffer SPL | 30 ml | 140 ml |
| Buffer PHC | 30 ml | 140 ml |
| Buffer GRP | 60 ml | 250 ml |
| Memoria intermedia RW1 | 50 ml | 250 ml |
| Buffer RW2 * | 20 ml | 2 X 50 ml |
| Agua libre de ARNasa | 15 ml | 30 ml |
Almacenamiento y estabilidad
- Condiciones de almacenaje: Kit components can be stored at room temperature (15–25°C) and remain stable for 18 meses en estas condiciones.
- Buffer SPL: May form precipitates at low temperatures. Dissolve precipitates by placing the Buffer SPL in a 55°C water bath.
- Buffer PHC: After receiving the product, store Buffer PHC at 2-8°C.
Reseñas
Aún no hay reseñas.