This product offers a convenient and rapid method to purify total DNA from a wide range of samples, making it ideal for various downstream analyses:
- Fast and Efficient: The protocol is streamlined for quick DNA extraction with minimal hands-on time.
- High-Quality DNA: The system isolates total DNA (genómico, viral, mitocondrial) with high purity, suitable for reliable PCR, PCR cuantitativa (qPCR), transferencia del sur, and viral DNA detection.
- Broad Sample Compatibility: The method effectively purifies DNA from a variety of sources, incluido:
- Tejido
- Células
- Sangre
- Saliva
- Swabs
- Blood Spots
- Semen
- Other clinical samples
- Downstream Applications: The purified DNA can be used directly in various research techniques, incluido:
- PCR (Reacción en cadena de la polimerasa)
- qPCR (PCR cuantitativa)
- Southern Blotting
- Viral DNA Detection
- Other DNA-based analyses
This solution simplifies DNA extraction while maintaining high quality, making it a valuable tool for researchers working in various fields of molecular biology, forense, and diagnostics.
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| Aplicaciones | PCR, qPCR, Detección de virus y pernos del sur., etc. |
| Método de purificación | Mini columna de giro |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Tejido, células, sangre, saliva, hisopos, manchas de sangre, semen, and other clinical samples |
| Cantidad de muestra | Solid tissue: 1-10mg; Sangre anticoagulante: 200µl |
| Volumen de elución | ≥20μl |
| Tiempo por carrera | 30 – 60 minutos |
| Volumen de transporte de líquido por columna | 800l |
| Rendimiento de unión de la columna | 100µg |
Principio
- Purification Method: Utilizes silica column purification for efficient nucleic acid extraction.
- Sample Lysis and Digestion: Samples are lysed and digested using lysate and protease, facilitating the release of DNA into the lysate.
- Nucleic Acid Adsorption: The lysed sample is transferred to an adsorption column, where nucleic acids selectively adsorb onto the membrane.
- Protein Removal: Proteins, which do not adsorb to the membrane, are removed through filtration, ensuring purity of the extracted nucleic acid.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted nucleic acid.
- Elution: Nucleic acid is finally eluted from the membrane using a low-salt buffer (10mm tris, pH 9.0, 0.5mmEDTA), resulting in efficient recovery of high-quality nucleic acid.
Ventajas
- High-quality DNA – meets a variety of downstream applications, incluyendo PCR, qPCR, digestión enzimática, hibridación, etc..
- Rápido – sin separación de leucocitos, organic extraction, or ethanol precipitation
- Simple – Todos los ácidos nucleicos se pueden obtener por digestión directa.
- Amplia aplicabilidad- Manejar una variedad de muestras líquidas.
Contenido del kit
| Contenido | D301802 | D301803 |
| Tiempos de purificación | 50 | 250 |
| Minicolumnas HiPure DNA I | 50 | 250 |
| 2Tubos de recolección de ml | 100 | 500 |
| ATL de búfer | 30 ml | 150 ml |
| Amortiguador AL | 30 ml | 150 ml |
| Memoria intermedia GW1 | 22 ml | 88 ml |
| Búfer GW2 | 12 ml | 50 ml |
| Proteinasa K | 24 mg | 120 mg |
| Tampón de disolución de proteasa | 1.8 ml | 10 ml |
| AE de búfer | 15 ml | 60 ml |
Almacenamiento y estabilidad
- Proteinase K Storage: Upon arrival, store Proteinase K at 2-8°C. Short-term storage (hasta 12 semanas) a temperatura ambiente (15-25°C) is acceptable without affecting performance.
- Remaining Kit Components Storage: Los componentes restantes del kit se pueden almacenar a temperatura ambiente. (15-25°C) and remain stable for at least 18 meses en estas condiciones.
- Whole Kit Storage: The entire kit can also be stored at 2-8°C. Sin embargo, in this case, buffers should be redissolved before use. Ensure all buffers are at room temperature when used for optimal performance.
Datos del experimento
