Introducción
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples. Alternatively, a miRNA-enriched fraction and a total RNA (>200nt) fraction can be purified separately. This Kit combines phenol/guanidina-based lysis of samples and silica membrane–based purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation miRNA and other small RNA molecules(18nt), from cultured cells and various animal and human tissues, using MagZol reagent and column |
| Aplicaciones | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Método de purificación | Mini columna de giro |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Tejidos animales, adherent cells, suspension cells, bacteria, etc. |
| Cantidad de muestra | Eukaryotic culture cells: ≤ 10^7, Tejido animal:<100mg, Yeast culture cells:<5 x10^7, Bacteria:<10^9 |
| Volumen de elución | ≥15μl |
| Tiempo por carrera | ≤40 minutos |
| Volumen de transporte de líquido por columna | 800l |
| Rendimiento de unión de la columna | 100µg |
Principio
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such asguanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein andother impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
This kit combines acid/guanidine (MagZol) extraction technology with glass fiber filter membrane purification, which can improve the extraction effect of complex samples and samples with low RNA content. After the sample is treated with MagZol reagent and chloroform, the supernatant is added with ethanol to provide appropriate binding conditions, then transferred to the purification column and centrifuged. Macromolecular RNA can be efficiently bound to the membrane. Collect the filtrate containing small RNA, add more ethanol to adjust the binding capacity of small RNA, the pollutants can be efFiciently washed away by second cleaning. Finally, the purified RNA was eluted by RNase free water.
Ventajas
- Alta calidad – one step extracción de ARN reagent combined with silica gel column can obtain the highest concentration
- Rápido – Se pueden extraer varias muestras en 40 minutos
- High applicability – samples including animals, plants, bacteria, células, etc..
- High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
Contenido del kit
| Contenido | R431002 | R431003 |
| Tiempos de purificación | 50 preparativos | 250 preparativos |
| Minicolumnas de ARN HiPure | 100 | 2 X 250 |
| 2Tubos de recolección de ml | 100 | 2 X 250 |
| MagZol Reagent | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 80 ml |
| Búfer RW2* | 20 ml | 2 X 50 ml |
| Agua libre de ARNasa | 10 ml | 30 ml |
Almacenamiento y estabilidad
MagZol Reagent should be stored at 2-8°C upon arrival. Sin embargo, almacenamiento a corto plazo (hasta 24 semanas) a temperatura ambiente (15-25°C) no afecta su rendimiento. Los componentes restantes del kit se pueden almacenar a temperatura ambiente. (15-25°C) y son estables durante al menos 18 meses en estas condiciones.
Reseñas
Aún no hay reseñas.